Anti-NSUN4 Antibody Picoband®

SKU: A09446-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: NSUN4

Specificity:

Background: NSUN4 belongsto a family of RNA 5-methylcytosine (m5C) methyltransferases(MTases).MTERF4 recruits NSUN4 to the large ribosomal subunit in a process crucial for mitochondrial ribosome biogenesis. NSUN4 is a dual function protein, which on the one hand is needed for 12S rRNA methylation, and on the other hand interacts withMTERF4 to facilitate mitoribosomal assembly. The calculatedMWof NSUN4 is 43 kDa, 29786-1-AP can detect the band around 40 kDa.

Immunogen: E.coli-derived human NSUN4 recombinant protein (Position: M1-K379). Human NSUN4 shares 86.3% amino acid (aa) sequence identity with mouse NSUN4.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Camara, Y., Asin-Cayuela, J., Park, C. B., Metodiev, M. D., Shi, Y., Ruzzenente, B., Kukat, C., Habermann, B., Wibom, R., Hultenby, K., Franz, T., Erdjument-Bromage, H., Tempst, P., Hallberg, B. M., Gustafsson, C. M., Larsson, N.-G. MTERF4 regulates translation by targeting the methyltransferase NSUN4 to the mammalian mitochondrial ribosome. Cell Metab. 13: 527-539, 2011. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 8/30/2013. 3. Spahr, H., Habermann, B., Gustafsson, C. M., Larsson, N.-G., Hallberg, B. M. Structure of the human MTERF4-NSUN4 protein complex that regulates mitochondrial ribosome biogenesis. Proc. Nat. Acad. Sci. 109: 15253-15258, 2012.

Uniprot ID: Q96CB9

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 40 kDa

Calculated Molecular Weight:

Gene ID: 387338

Protein Name: 5-cytosine rRNA methyltransferase NSUN4

Gene Full Name: NOP2/Sun RNA methyltransferase 4

Synonyms: NSUN4; RP4 603I14.2