Anti-NSFL1C Antibody Picoband®

SKU: A08116-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,IF,ICC,WB,Flow Cytometry

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg /1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: NSFL1C

Specificity:

Background: NSFL1 cofactor p47 is a protein that in humans is encoded by the NSFL1C gene. N-ethylmaleimide-sensitive factor (NSF) and valosin-containing protein (p97) are two ATPases known to be involved in transport vesicle/target membrane fusion and fusions between membrane compartments. A trimer of the protein encoded by this gene binds a hexamer of cytosolic p97 and is required for p97-mediated regrowth of Golgi cisternae from mitotic Golgi fragments. Alternative splicing results in multiple transcript variants. A related pseudogene has been identified on chromosome 8.

Immunogen: E.coli-derived human NSFL1C recombinant protein (Position: E20-E291).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Kondo, H., Rabouille, C., Newman, R., Levine, T. P., Pappin, D., Freemont, P., Warren, G. p47 is a cofactor for p97-mediated membrane fusion. Nature 388: 75-78, 1997. 2. Zhang, Q.-H., Ye, M., Wu, X.-Y., Ren, S.-X., Zhao, M., Zhao, C.-J., Fu, G., Shen, Y., Fan, H.-Y., Lu, G., Zhong, M., Xu, X.-R., and 9 others. Cloning and functional analysis of cDNAs with open reading frames for 300 previously undefined genes expressed in CD34+ hematopoietic stem/progenitor cells. Genome Res. 10: 1546-1560, 2000.

Uniprot ID: Q9UNZ2

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 41 kDa

Calculated Molecular Weight: 17136 MW

Gene ID: 55968

Protein Name: NSFL1 cofactor p47

Gene Full Name: NSFL1 cofactor

Synonyms: NSFL1 (p97) cofactor (p47); NSFL1 cofactor p47; NSFL1C; p47; p97 cofactor p47; UBX1; UBXD10; UBXN2C