Anti-Nr0b2 Antibody Picoband®

SKU: A03866-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Mouse,Rat

Applications: ELISA,Flow Cytometry,IF,ICC,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Mouse
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Mouse
ELISA, 0.1-0.5 μg/ml, -

Gene Name: Nr0b2

Specificity:

Background: The small heterodimer partner (SHP) also known as NR0B2 (nuclear receptor subfamily 0, group B, member 2) is a protein that in humans is encoded by the NR0B2 gene. This gene encodes a member of the nuclear hormone receptor family of proteins. The encoded orphan receptor lacks a DNA binding domain but modulates gene expression by interacting with other hormone receptors to competitively inhibit binding of coactivators while also acting as a transcriptional corepressor. The encoded receptor may regulate bile acid synthesis and sexual maturation in males. Homozygous knockout mice exhibit accumulation of bile acids, reduced levels of low density lipoprotein cholesterol (LDL-C), and in male mice, earlier testicular maturation.

Immunogen: E.coli-derived mouse Nr0b2 recombinant protein (Position: A54-R219).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Echwald, S. M., Andersen, K. L., Sorensen, T. I. A., Larsen, L. H., Andersen, T., Tonooka, N., Tomura, H., Takeda, J., Pedersen, O. Mutation analysis of NR0B2 among 1545 Danish men identifies a novel c.278G-A (p.G93D) variant with reduced functional activity. Hum. Mutat. 24: 381-387, 2004. 2. Goodwin, B., Jones, S. A., Price, R. R., Watson, M. A., McKee, D. D., Moore, L. B., Galardi, C., Wilson, J. G., Lewis, M. C., Roth, M. E., Maloney, P. R., Willson, T. M., Kliewer, S. A. A regulatory cascade of the nuclear receptors FXR, SHP-1, and LRH-1 represses bile acid biosynthesis. Molec. Cell 6: 517-526, 2000. 3. Iyer, A. K., Zhang, Y.-H., McCabe, E. R. B. Dosage-sensitive sex reversal adrenal hypoplasia congenita critical region on the X chromosome, gene 1 (DAX1) (NR0B1) and small heterodimer partner (SHP) (NR0B2) form homodimers individually, as well as DAX1-SHP heterodimers. Molec. Endocr. 20: 2326-2342, 2006.

Uniprot ID: Q62227

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 28 kDa

Calculated Molecular Weight: 127459 MW

Gene ID: 23957

Protein Name: Nuclear receptor subfamily 0 group B member 2

Gene Full Name: nuclear receptor subfamily 0, group B, member 2

Synonyms: Shp; Nr0b2; Small heterodimer partner