Anti-NCAPH Antibody Picoband®

SKU: A09559-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: NCAPH

Specificity:

Background: Condensin complex subunit 2 also known as chromosome-associated protein H (CAP-H) or non-SMC condensin I complex subunit H (NCAPH) is a protein that in humans is encoded by the NCAPH gene. This gene encodes a member of the barr gene family and a regulatory subunit of the condensin complex. This complex is required for the conversion of interphase chromatin into condensed chromosomes. The protein encoded by this gene is associated with mitotic chromosomes, except during the early phase of chromosome condensation. During interphase, the protein has a distinct punctate nucleolar localization. Alternatively spliced transcript variants encoding different proteins have been described.

Immunogen: E.coli-derived human NCAPH recombinant protein (Position: R91-K721).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Aono, N., Sutani, T., Tomonaga, T., Mochida, S., Yanagida, M. Cnd2 has dual roles in mitotic condensation and interphase. Nature 417: 197-202, 2002. 2. Bhat, M. A., Philp, A. V., Glover, D. M., Bellen, H. J. Chromatid segregation at anaphase requires the barren product, a novel chromosome-associated protein that interacts with topoisomerase II. Cell 87: 1103-1114, 1996. 3. Cabello, O. A., Baldini, A., Bhat, M., Bellen, H., Belmont, J. W. Localization of BRRN1, the human homologue of Drosophila barr, to 2q11.2. Genomics 46: 311-313, 1997.

Uniprot ID: Q15003

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 100 kDa

Calculated Molecular Weight:

Gene ID: 23397

Protein Name: Condensin complex subunit 2

Gene Full Name: non-SMC condensin I complex subunit H

Synonyms: Barren homolog protein 1; BRRN; BRRN1; CAP H; CAPH; CND2; Condensin complex subunit 2; HCAP H; KIAA0074; NCAPH; XCAP H homolog