Anti-Myosin Light Chain 2/MLC-2V/MYL2 Antibody Picoband®

SKU: A03215-3

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Monkey,Mouse,Rat

Applications: WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Monkey, Mouse, Rat

Gene Name: MYL2

Specificity:

Background: Myosin regulatory light chain 2, ventricular/cardiac muscle isoform (MLC-2) also known as the regulatory light chain of myosin (RLC) is a protein that in humans is encoded by the MYL2 gene. This gene encodes a major sarcomeric protein in mammalian striated muscle. The encoded protein plays a role in embryonic heart muscle structure and function, while phosphorylation of the encoded protein is involved in cardiac myosin cycling kinetics, torsion and function in adults. Mutations in this gene are associated with hypertrophic cardiomyopathy 10 and infant-onset myopathy.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Myosin Light Chain 2/MLC-2V/MYL2, which shares 95.7% amino acid (aa) sequence identity with both mouse and rat Myosin Light Chain 2/MLC-2V/MYL2.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Barth, P. G., Wanders, R. J. A., Ruitenbeek, W., Roe, C., Scholte, H. R., van der Harten, H., van Moorsel, J., Duran, M., Dingemans, K. P. Infantile fibre type disproportion, myofibrillar lysis and cardiomyopathy: a disorder in three unrelated Dutch families. Neuromusc. Disord. 8: 296-304, 1998. 2. Davis, J. S., Hassanzadeh, S., Winitsky, S., Lin, H., Satorius, C., Vemuri, R., Aletras, A. H., Wen, H., Epstein, N. D. The overall pattern of cardiac contraction depends on a spatial gradient of myosin regulatory light chain phosphorylation. Cell 107: 631-641, 2001. 3. Flavigny, J., Richard, P., Isnard, R., Carrier, L., Charron, P., Bonne, G., Forissier, J.-F., Desnos, M., Dubourg, O., Komajda, M., Schwartz, K., Hainque, B. Identification of two novel mutations in the ventricular regulatory myosin light chain gene (MYL2) associated with familial and classical forms of hypertrophic cardiomyopathy. J. Molec. Med. 76: 208-214, 1998.

Uniprot ID: P10916

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 19 kDa

Calculated Molecular Weight: 19 kDa

Gene ID: 4633

Protein Name: Myosin regulatory light chain 2, ventricular/cardiac muscle isoform

Gene Full Name: myosin light chain 2

Synonyms: MLC 2; MLC 2v; MLC2; MLC2V; MYL2; Myosin Light Chain 2; Myosin Light Chain 2/MLC-2V