Anti-MYOF Antibody Picoband®

SKU: A04580-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
ELISA, 0.1-0.5 μg/ml

Gene Name: MYOF

Specificity:

Background: Mutations in dysferlin, a protein associated with the plasma membrane, can cause muscle weakness that affects both proximal and distal muscles. The protein encoded by this gene is a type II membrane protein that is structurally similar to dysferlin. It is a member of the ferlin family and associates with both plasma and nuclear membranes. The protein contains C2 domains that play a role in calcium-mediated membrane fusion events, suggesting that it may be involved in membrane regeneration and repair. Two transcript variants encoding different isoforms have been found for this gene. Other possible variants have been detected, but their full-length nature has not been determined.

Immunogen: E.coli-derived human MYOF recombinant protein (Position: E175-E2003). Human MYOF shares 93.9% amino acid (aa) sequence identity with mouse MYOF.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Ariano, A., D'Apolito, M., Bova, M., Bellanti, F., Loffredo, S., D'Andrea, G., Intrieri, M., Petraroli, A., Maffione, A. B., Spadaro, G., Santacroce, R., Margaglione, M. A myoferlin gain-of-function variant associates with a new type of hereditary angioedema. (Letter) Allergy 75: 2989-2992, 2020. 2. Britton, S., Freeman, T., Vafiadaki, E., Keers, S., Harrison, R., Bushby, K., Bashir, R. The third human FER-1-like protein is highly similar to dysferlin. Genomics 68: 313-321, 2000. 3. Davis, D. B., Delmonte, A. J., Ly, C. T., McNally, E. M. Myoferlin, a candidate gene and potential modifier of muscular dystrophy. Hum. Molec. Genet. 9: 217-226, 2000.

Uniprot ID: Q9NZM1

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 250 kDa

Calculated Molecular Weight: 235 kDa

Gene ID: 26509

Protein Name: Myoferlin

Gene Full Name: myoferlin

Synonyms: Myoferlin; KIAA1207; Fer-1-like protein 3; FER1L3; Fer 1 like protein 3