Anti-MYLK3 Antibody Picoband®

SKU: A09180-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: MYLK3

Specificity:

Background: Myosin light chain kinase 3 also known as MYLK3, is an enzyme which in humans is encoded by the MYLK3 gene. Phosphorylation of cardiac myosin heavy chains (see MYH7B, MIM 609928) and light chains (see MYL2, MIM 160781) by a kinase, such as MYLK3, potentiates the force and rate of cross-bridge recruitment in cardiac myocytes.

Immunogen: E.coli-derived human MYLK3 recombinant protein (Position: E34-K812). Human MYLK3 shares 68.8% and 66.8% amino acid (aa) sequence identity with mouse and rat MYLK3, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Chan, J. Y., Takeda, M., Briggs, L. E., Graham, M. L., Lu, J. T., Horikoshi, N., Weinberg, E. O., Aoki, H., Sato, N., Chien, K. R., Kasahara, H. Identification of cardiac-specific myosin light chain kinase. Circ. Res. 102: 571-580, 2008. 2. Hartz, P. A. Personal Communication. Baltimore, Md. 6/30/2008. 3. Seguchi, O., Takashima, S., Yamazaki, S., Asakura, M., Asano, Y., Shintani, Y., Wakeno, M., Minamino, T., Kondo, H., Furukawa, H., Nakamuru, K., Naito, A., Takahashi, T., Ohtsuka, T., Kawakami, K., Isomura, T., Kitamura, S., Tomoike, H., Mochizuki, N., Kitakaze, M. A cardiac myosin light chain kinase regulates sarcomere assembly in the vertebrate heart. J. Clin. Invest. 117: 2812-2824, 2007.

Uniprot ID: Q32MK0

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 100 kDa

Calculated Molecular Weight:

Gene ID: 91807

Protein Name: Myosin light chain kinase 3

Gene Full Name: myosin light chain kinase 3

Synonyms: caMLCK; MLCK; MLCK2; MYLK3; myosin light chain kinase 3