Anti-MPST Antibody Picoband®

SKU: A04609-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: MPST

Specificity:

Background: This protein encoded by this gene catalyzes the transfer of a sulfur ion from 3-mercaptopyruvate to cyanide or other thiol compounds. It may be involved in cysteine degradation and cyanide detoxification. There is confusion in literature between this protein (mercaptopyruvate sulfurtransferase, MPST), which appears to be cytoplasmic, and thiosulfate sulfurtransferase (rhodanese, TST, GeneID:7263), which is a mitochondrial protein. Deficiency in MPST activity has been implicated in a rare inheritable disorder known as mercaptolactate-cysteine disulfiduria (MCDU). Alternatively spliced transcript variants encoding same or different isoforms have been identified for this gene.

Immunogen: E.coli-derived human MPST recombinant protein (Position: A2-H297).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Aita, N., Ishii, K., Akamatsu, Y., Ogasawara, Y., Tanabe, S. Cloning and expression of human liver rhodanese cDNA. Biochem. Biophys. Res. Commun. 231: 56-60, 1997. 2. Billaut-Laden, I., Rat, E., Allorge, D., Crunelle-Thibaut, A., Cauffiez, C., Chevalier, D., Lo-Guidice, J.-M., Broly, F. Evidence for a functional genetic polymorphism of the human mercaptopyruvate sulfurtransferase (MPST), a cyanide detoxification enzyme. Toxicol. Lett. 165: 101-111, 2006. 3. Hartz, P. A. Personal Communication. Baltimore, Md. 12/8/2009.

Uniprot ID: P25325

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 33 kDa

Calculated Molecular Weight:

Gene ID: 4357

Protein Name: 3-mercaptopyruvate sulfurtransferase

Gene Full Name: mercaptopyruvate sulfurtransferase

Synonyms: MPST; TST2; MST; TUM1