Anti-MIRO2/RHOT2 Antibody Picoband®

SKU: A08801-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,Flow Cytometry,IP,IF,IHC,ICC,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunoprecipitation, 0.5-2 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: RHOT2

Specificity:

Background: This gene encodes a member of the Rho family of GTPases. The encoded protein is localized to the outer mitochondrial membrane and plays a role in mitochondrial trafficking and fusion-fission dynamics.

Immunogen: E.coli-derived human MIRO2/RHOT2 recombinant protein (Position: D321-V615). Human MIRO2/RHOT2 shares 78.5% and 76.3% amino acid (aa) sequence identity with mouse and rat MIRO2/RHOT2, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Fransson, A., Ruusala, A., Aspenstrom, P. Atypical Rho GTPases have roles in mitochondrial homeostasis and apoptosis. J. Biol. Chem. 278: 6495-6502, 2003. 2. Shan, Y., Hexige, S., Guo, Z., Wan, B., Chen, K., Chen, X., Ma, L., Huang, C., Zhao, S., Yu, L. Cloning and characterization of the mouse Arht2 gene which encodes a putative atypical GTPase. Cytogenet. Genome Res. 106: 91-97, 2004.

Uniprot ID: Q8IXI1

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 80 kDa

Calculated Molecular Weight: 68 kDa

Gene ID: 89941

Protein Name: Mitochondrial Rho GTPase 2

Gene Full Name: ras homolog family member T2

Synonyms: ARHT2; C16orf39; hMiro 2; MIRO 2; miro2; Mitochondrial Rho GTPase 2; RASL; RHOT2