Anti-MED14 Antibody Picoband®

SKU: A04799-2

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: ELISA,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human
ELISA, 0.1-0.5μg/ml

Gene Name: MED14

Specificity: No cross reactivity with other proteins.

Background: Mediator of RNA polymerase II transcription subunit 14 is an enzyme that in humans is encoded by the MED14 gene. The activation of gene transcription is a multistep process that is triggered by factors that recognize transcriptional enhancer sites in DNA. These factors work with co-activators to transcriptional initiation by the RNA polymerase II apparatus. The protein encoded by this gene is a subunit of the CRSP (cofactor required for SP1 activation) complex, which, along with TFIID, is required for efficient activation by SP1. This protein is also a component of other multisubunit complexes e.g. thyroid hormone receptor- (TR-) associated proteins which interact with TR and facilitate TR function on DNA templates in conjunction with initiation factors and cofactors. This protein contains a bipartite nuclear localization signal. This gene is known to escape chromosome X-inactivation.

Immunogen: E. coli-derived human MED14 recombinant protein (Position: D181-D375).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Ryu S, Zhou S, Ladurner AG, Tjian R (Feb 1999). The transcriptional cofactor complex CRSP is required for activity of the enhancer-binding protein Sp1. Nature. 397 (6718): 446–50. 2. Yoshikawa H, Fujiyama A, Nakai K, Inazawa J, Matsubara K (Aug 1998). Detection and isolation of a novel human gene located on Xp11.2-p11.4 that escapes X-inactivation using a two-dimensional DNA mapping method. Genomics. 49 (2): 237–46.

Uniprot ID: O60244

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 160 kDa

Calculated Molecular Weight: 160 kDa

Gene ID: 9282

Protein Name: Mediator of RNA polymerase II transcription subunit 14

Gene Full Name: mediator complex subunit 14

Synonyms: mediator complex subunit 14; CRSP150; CRSP2; CSRP; CXorf4; DRIP150; EXLM1; RGR1; TRAP170; MED14