Anti-MCM9 Antibody Picoband®

SKU: A07226-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, Human

Gene Name: MCM9

Specificity:

Background: The protein encoded by this gene is a member of the mini-chromosome maintenance (MCM) protein family that are essential for the initiation of eukaryotic genome replication. Binding of this protein to chromatin has been shown to be a pre-requisite for recruiting the MCM2-7 helicase to DNA replication origins. This protein also binds, and is a positive regulator of, the chromatin licensing and DNA replication factor 1, CDT1.

Immunogen: E.coli-derived human MCM9 recombinant protein (Position: M1-L644). Human MCM9 shares 92.7% and 93% amino acid (aa) sequence identity with mouse and rat MCM9, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Fauchereau, F., Shalev, S., Chervinsky, E., Beck-Fruchter, R., Legois, B., Fellous, M., Caburet, S., Veitia, R. A. A non-sense MCM9 mutation in a familial case of primary ovarian insufficiency. Clin. Genet. 89: 603-607, 2016. 2. Lutzmann, M., Grey, C., Traver, S., Ganier, O., Maya-Mendoza, A., Ranisavljevic, N., Bernex, F., Nishiyama, A., Montel, N., Gavois, E., Forichon, L., de Massy, B., Mechali, M. MCM8- and MCM9-deficient mice reveal gametogenesis defects and genome instability due to impaired homologous recombination. Molec. Cell 47: 523-534, 2012. 3. Lutzmann, M., Maiorano, D., Mechali, M. Identification of full genes and proteins of MCM9, a novel, vertebrate-specific member of the MCM2-8 protein family. Gene 362: 51-56, 2005.

Uniprot ID: Q9NXL9

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 127 kDa

Calculated Molecular Weight: 127 kDa

Gene ID: 254394

Protein Name: DNA helicase MCM9

Gene Full Name: minichromosome maintenance 9 homologous recombination repair factor

Synonyms: MCM9; C6orf61; MCMDC1; DNA helicase MCM9; hMCM9; EC 3.6.4.12; Mini-chromosome maintenance deficient domain-containing protein 1; Minichromosome maintenance 9