Anti-MARK3 Antibody Picoband®

SKU: A05355-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: MARK3

Specificity:

Background: MAP/microtubule affinity-regulating kinase 3 is an enzyme that in humans is encoded by the MARK3 gene. The protein encoded by this gene is activated by phosphorylation and in turn is involved in the phosphorylation of tau proteins MAP2 and MAP4. Several transcript variants encoding different isoforms have been found for this gene.

Immunogen: E.coli-derived human MARK3 recombinant protein (Position: H311-D648).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Ansar, M., Chung, H., Waryah, Y. M., Makrythanasis, P., Falconnet, E., Rao, A. R., Guipponi, M., Narsani, A. K., Fingerhut, R., Santoni, F. A., Ranza, E., Waryah, A. M., Bellen, H. J., Antonarakis, S. E. Visual impairment and progressive phthisis bulbi caused by recessive pathogenic variant in MARK3. Hum. Molec. Genet. 27: 2703-2711, 2018. 2. Lennerz, J. K., Hurov, J. B., White, L. S., Lewandowski, K. T., Prior, J. L., Planer, G. J., Gereau, R. W., IV, Piwnica-Worms, D., Schmidt, R. E., Piwnica-Worms, H. Loss of Par-1a/MARK3/C-TAK1 kinase leads to reduced adiposity, resistance to hepatic steatosis, and defective gluconeogenesis. Molec. Cell. Biol. 30: 5043-5056, 2010. 3. Muller, J., Ory, S., Copeland, T., Piwnica-Worms, H., Morrison, D. K. C-TAK1 regulates Ras signaling by phosphorylating the MAPK scaffold, KSR1. Molec. Cell 8: 983-993, 2001.

Uniprot ID: P27448

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 80 kDa

Calculated Molecular Weight: 39411 MW

Gene ID: 4140

Protein Name: MAP/microtubule affinity-regulating kinase 3

Gene Full Name: microtubule affinity regulating kinase 3

Synonyms: C TAK1; CTAK1; ELKL motif kinase 2; EMK 2; EMK2; KP78; MARK3; PAR1A; Protein kinase STK10; Ser/Thr protein kinase PAR 1