Anti-MADD Antibody Picoband®

SKU: A02030-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: MADD

Specificity:

Background: MAP kinase-activating death domain protein is an enzyme that in humans is encoded by the MADD gene. Tumor necrosis factor alpha (TNF-alpha) is a signaling molecule that interacts with one of two receptors on cells targeted for apoptosis. The apoptotic signal is transduced inside these cells by cytoplasmic adaptor proteins. The protein encoded by this gene is a death domain-containing adaptor protein that interacts with the death domain of TNF-alpha receptor 1 to activate mitogen-activated protein kinase (MAPK) and propagate the apoptotic signal. It is membrane-bound and expressed at a higher level in neoplastic cells than in normal cells. Several transcript variants encoding different isoforms have been described for this gene.

Immunogen: E.coli-derived human MADD recombinant protein (Position: K5-Y329).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Al-Zoubi, A. M., Efimova, E. V., Kaithamana, S., Martinez, O., El-Azami El-Idrissi, M., Dogan, R. E., Prabhakar, B. S. Contrasting effects of IG20 and its splice isoforms, MADD and DENN-SV, on tumor-necrosis factor alpha-induced apoptosis and activation of caspase-8 and -3. J. Biol. Chem. 276: 47202-47211, 2001. 2. Anazi, S., Maddirevula, S., Salpietro, V., Asi, Y. T., Alsahli, S., Alhashem, A., Shamseldin, H. E., AlZahrani, F., Patel, N., Ibrahim, N., Abdulwahab, F. M., Hashem, M., and 31 others. Expanding the genetic heterogeneity of intellectual disability. Hum. Genet. 136: 1419-1429, 2017. Note: Erratum: Hum. Genet. 137: 105-109, 2018. 3. Chow, V. T. K., Lee, S. S. DENN, a novel human gene differentially expressed in normal and neoplastic cells. DNA Seq. 6: 263-273, 1996.

Uniprot ID: Q8WXG6

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 240 kDa

Calculated Molecular Weight: 183 kDa

Gene ID: 8567

Protein Name: MAP kinase-activating death domain protein

Gene Full Name: MAP kinase activating death domain

Synonyms: DENN; FLJ35600; FLJ36300; IG20; KIAA0358; MADD; Rab3 GDP/GTP exchange factor; RAB3GEP