Anti-LRRC75A Antibody Picoband®

SKU: A18461-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: LRRC75A

Specificity:

Background: Long noncoding RNAs (lncRNAs) play multiple key roles during inflammatory processes. A novel lncRNA identified by the high-throughput sequencing analysis was found significantly down-regulated in Escherichia coli-introduced cell model of bovine mastitis. Given that this lncRNA consists of the antisense of leucine-rich repeat-containing protein 75A (LRRC75A), it was named LRRC75A antisense lncRNA1 (LRRC75A-AS1). The expression of LRRC75A-AS1 was down-regulated in bovine mammary epithelial cells and mammary tissues under inflammatory condition. Knockout (KO) of LRRC75A-AS1 by CRISPR-Cas9 system in bovine mammary alveolar cell-T (MAC-T) cell line could enhance expressions of tight junction (TJ) proteins Claudin-1, Occludin and ZO-1, reduce cell monolayer permeability, and inhibit Staphylococcus aureus adhesion and invasion. Meanwhile, it also down-regulated expressions of inflammatory factors and attenuated activation of NF-κB pathway. Similarly, knockdown of LRRC75A caused the changes as LRRC75A-AS1 KO did, while overexpression of LRRC75A enabled the opposite effects. TJ of epithelioid cells barriers the pathogenic microorganisms outside during inflammation, in which LRRC75A-AS1 can regulate the expression of TJ proteins through LRRC75A, affecting the development of inflammation.

Immunogen: E.coli-derived human LRRC75A recombinant protein (Position: H52-Q292).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Miura, T., Kouno, T., Takano, M., Kuroda, T., Yamamoto, Y., Kusakawa, S., ... & Sato, Y. (2023). Single-Cell RNA-Seq Reveals LRRC75A-Expressing Cell Population Involved in VEGF Secretion of Multipotent Mesenchymal Stromal/Stem Cells Under Ischemia. Stem Cells Translational Medicine, 12(6), 379-390. 2. Yan, D., He, D., He, S., Chen, X., Fan, Z., & Chen, R. (2011). Identification and analysis of intermediate size noncoding RNAs in the human fetal brain. PLoS One, 6(7), e21652. 3. Rose, J. E., Behm, F. M., Drgon, T., Johnson, C., & Uhl, G. R. (2010). Personalized smoking cessation: interactions between nicotine dose, dependence and quit-success genotype score. Molecular medicine, 16(7), 247-253.

Uniprot ID: Q8NAA5

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 36 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 388341

Protein Name: Leucine-rich repeat-containing protein 75A

Gene Full Name: leucine rich repeat containing 75A

Synonyms: LRRC75A; C17orf76; FAM211A; Leucine-rich repeat-containing protein 75A; Leucine-rich repeat-containing protein FAM211A