Anti-Liver Carboxylesterase 1/CES1 Antibody Picoband® (monoclonal, 3F10)

SKU: M01741-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry

Application Details: Western blot, 0.1-0.25μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human

Gene Name: CES1

Specificity:

Background: Liver carboxylesterase 1 also known as carboxylesterase 1 (CES1, hCE-1 or CES1A1) is an enzyme that in humans is encoded by the CES1 gene. This gene encodes a member of the carboxylesterase large family. The family members are responsible for the hydrolysis or transesterification of various xenobiotics, such as cocaine and heroin, and endogenous substrates with ester, thioester, or amide bonds. They may participate in fatty acyl and cholesterol ester metabolism, and may play a role in the blood-brain barrier system. This enzyme is the major liver enzyme and functions in liver drug clearance. Mutations of this gene cause carboxylesterase 1 deficiency. Three transcript variants encoding three different isoforms have been found for this gene.

Immunogen: E. coli-derived human Liver Carboxylesterase 1/CES1 recombinant protein (Position: E99-A206).

Clonality: Monoclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Entrez Gene: CES1 carboxylesterase 1 (monocyte/macrophage serine esterase 1). 2. Markey GM (Feb 2011). Carboxylesterase 1 (Ces1): from monocyte marker to major player. J Clin Pathol. 64 (2): 107–9. 3. Zhu HJ, Appel DI, Johnson JA, Chavin KD, Markowitz JS (Jan 2009). Role of carboxylesterase 1 and impact of natural genetic variants on the hydrolysis of trandolapril. Biochem Pharmacol. 77 (7): 1266–72.

Uniprot ID: P23141

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG2b

Phospho_site:

Clone Number: Clone: 3F10

Observed Molecular Weight: 63 kDa

Calculated Molecular Weight:

Gene ID: 1066

Protein Name: Liver carboxylesterase 1

Gene Full Name: carboxylesterase 1

Synonyms: ACAT; Brain carboxylesterase hBr1; CE1; CEH; CES1; Cocaine carboxylesterase; Egasyn; HMSE; HMSE1; Liver carboxylesterase 1; PCE 1; REH; Retinyl ester hydrolase; Serine esterase 1; SES1; TGH; Triacylglycerol hydrolase