Anti-IWS1 Antibody Picoband®

SKU: A05958-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: IWS1

Specificity:

Background: Protein IWS1 homolog also known as interacts with Spt6 (IWS1) is a protein that in humans is encoded by the IWS1 gene. Various steps in gene expression, such as mRNA processing, surveillance, export, and synthesis are coupled to transcription elongation). The C-terminal domain (CTD) of the large subunit of RNA polymerase II plays an important role in the integration of these different steps. IWS1 interacts with Spt6, a CTD-binding transcription elongation factor and H3 chaperone. IWS1 also recruits another CTD-binding protein, HYPB/Setd2 histone methyltransferase, to the RNA polymerase II complex for elongation-coupled H3K36 trimethylation. Thus, IWS1 links Spt6 and HYPB/Setd2 in a large complex and regulates mRNA synthesis and histone methylation at the co-transcriptional level .

Immunogen: E.coli-derived human IWS1 recombinant protein (Position: R547-E771).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Yoh, S. M. , Cho, H. , Pickle, L. , Evans, R. M. , & Jones, K. A. . (2007). The spt6 sh2 domain binds ser2-p rnapii to iws1-dependent mrna splicing and export. Genes & Development, 21(2), 160. 2. Yoh, S. M. , Lucas, J. S. , & Jones, K. A. . (2008). The iws1:spt6:ctd complex controls cotranscriptional mrna biosynthesis and hypb/setd2-mediated histone h3k36 methylation. Genes Dev, 22(24), 3422-3434.

Uniprot ID: Q96ST2

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 140 kDa

Calculated Molecular Weight: 80420 MW

Gene ID: 55677

Protein Name: Protein IWS1 homolog

Gene Full Name: interacts with SUPT6H, CTD assembly factor 1

Synonyms: IWS1; IWS1 like protein; IWS1L; Protein IWS1 homolog