Anti-IL8/CXCL8 Antibody Picoband®

SKU: PA1353

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: IHC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human

Gene Name: IL8

Specificity: No cross reactivity with other proteins.

Background: Interleukin-8 (IL-8) is a chemokine produced by macrophages and other cell types such as epithelial cells. It is also synthesized by endothelial cells, which store IL-8 in their storage vesicles, the Weibel-Palade bodies. In humans, the interleukin-8 protein is encoded by the IL8 gene. Interleukin-8 (IL8) is a member of the CXC chemokine family (Hull et al., 2001). IL-8 is believed to play a role in the pathogenesis of bronchiolitis, a common respiratory tract disease caused by viral infection. This gene and other ten members of the CXC chemokine gene family form a chemokine gene cluster in a region mapped to chromosome 4q. The genes for IL8 have been co-localized on a 335-kb genomic fragment.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human IL8.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Wolff B, Burns AR, Middleton J, Rot A (November 1998). Endothelial cell memory of inflammatory stimulation: human venular endothelial cells store interleukin 8 in Weibel-Palade bodies. 2. Utgaard JO, Jahnsen FL, Bakka A, Brandtzaeg P, Haraldsen G (November 1998). Rapid secretion of prestored interleukin 8 from Weibel-Palade bodies of microvascular endothelial cells. 3. Modi WS, Dean M, Seuanez HN, Mukaida N, Matsushima K, O'Brien SJ (January 1990). Monocyte-derived neutrophil chemotactic factor (MDNCF/IL-8) resides in a gene cluster along with several other members of the platelet factor 4 gene superfamily. 4. Entrez Gene: IL8 interleukin 8. 5. Köhidai L, Csaba G (July 1998). Chemotaxis and chemotactic selection induced with cytokines (IL-8, RANTES and TNF-alpha) in the unicellular Tetrahymena pyriformis. 6. O'Donovan, N., Galvin, M., Morgan, J. G. Physical mapping of the CXC chemokine locus on human chromosome 4. Cytogenet.

Uniprot ID: P10145

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 60 kDa

Calculated Molecular Weight: 11098 MW

Gene ID: 3576

Protein Name: Interleukin-8

Gene Full Name: C-X-C motif chemokine ligand 8

Synonyms: C X C motif chemokine 8; CXCL8; CXCL8; IL8; CXCL8/IL-8; Emoctakin; GCP 1; GCP1; IL 8; IL 8(1 77); IL8; IL-8; IL8/NAP1 form I Interleukin 8; IL8/NAP1 form II; IL8/NAP1 form VI; interleukin 8; MDNCF; MDNCF b IL 8(5 77) IL 8(6 77); NAF; NAF IL 8(7 77); NAP 1; NAP1; Neutrophil activating factor; Protein 3 10C