Anti-IARS2 Antibody Picoband®

SKU: A09580-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,Flow Cytometry,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: IARS2

Specificity:

Background: Aminoacyl-tRNA synthetases catalyze the aminoacylation of tRNA by their cognate amino acid. Because of their central role in linking amino acids with nucleotide triplets contained in tRNAS, aminoacyl-tRNA synthetases are thought to be among the first proteins that appeared in evolution. Two forms of isoleucine-tRNA synthetase exist, a cytoplasmic form and a mitochondrial form. This gene encodes the mitochondrial isoleucine-tRNA synthetase which belongs to the class-I aminoacyl-tRNA synthetase family.

Immunogen: E.coli-derived human OTUB1 recombinant protein (Position: K10-K271). Human OTUB1 shares 99.6% amino acid (aa) sequence identity with both mouse and rat OTUB1.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Bonnefond, L., Fender, A., Rudinger-Thirion, J., Giege, R., Florentz, C., Sissler, M. Toward the full set of human mitochondrial aminoacyl-tRNA synthetases: characterization of AspRS and TyrRS. Biochemistry 44: 4805-4816, 2005. 2. Liberfarb, R. M., Jackson, A. H., Eavey, R. D., Robb, R. M. Unique hereditary sensory and autonomic neuropathy with growth hormone deficiency. J. Child Neurol. 8: 271-276, 1993. 3. Moosa, S., Haagerup, A., Gregersen, P. A., Petersen, K. K., Altmuller, J., Thiele, H., Nurnberg, P., Cho, T.-J., Kim, O.-H., Nishimura, G., Wollnik, B., Vogel, I. Confirmation of CAGSSS syndrome as a distinct entity in a Danish patient with a novel homozygous mutation in IARS2. Am. J. Med. Genet. 173: 1102-1108, 2017.

Uniprot ID: Q9NSE4

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 114 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 55699

Protein Name: Isoleucine--tRNA ligase, mitochondrial

Gene Full Name: isoleucyl-tRNA synthetase 2, mitochondrial

Synonyms: IARS2; Isoleucine--tRNA ligase, mitochondrial; Isoleucyl-tRNA synthetase; IleRS