Anti-HSD3B1 Antibody Picoband® (monoclonal, 3F3C/7F9C8)

SKU: M02856

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,ICC,IF

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human

Gene Name: HSD3B1

Specificity:

Background: HSD3B1 is a human gene that encodes for a 3beta-hydroxysteroid dehydrogenase/delta(5)-delta(4)isomerase type I or hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1. It is mapped to 1p12. The protein encoded by this gene is an enzyme that catalyzes the oxidative conversion of delta-5-3-beta-hydroxysteroid precursors into delta-4-ketosteroids, which leads to the production of all classes of steroid hormones. The encoded protein also catalyzes the interconversion of 3-beta-hydroxy- and 3-keto-5-alpha-androstane steroids.

Immunogen: E.coli-derived human HSD3B1 recombinant protein (Position: Q17-Q373).

Clonality: Monoclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Taneja SS (January 2017). Re: HSD3B1 and Resistance to Androgen-Deprivation Therapy in Prostate Cancer: A Retrospective, Multicohort Study. The Journal of Urology. 197 (1): 150. 2. Hearn, Jason (October 2016). HSD3B1 and resistance to androgen-deprivation therapy in prostate cancer: a retrospective, multicohort study. Lancet Oncology. 17 (10): 1435–1444. 3. Russell AJ, Gaffney D, Gardiner MT, Nickson DA, Sutcliffe RG (March 1991). Synonymous polymorphism in the coding sequence of human 3-beta hydroxysteroid-5-ene dehydrogenase (HSD). Nucleic Acids Research. 19 (5): 1172.

Uniprot ID: P14060

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG2b

Phospho_site:

Clone Number: Clone: 3F3C/7F9C8

Observed Molecular Weight: 42 kDa

Calculated Molecular Weight:

Gene ID: 3283

Protein Name: 3 beta-hydroxysteroid dehydrogenase/Delta 5-->4-isomerase type 1

Gene Full Name: hydroxy-delta-5-steroid dehydrogenase, 3 beta- and steroid delta-isomerase 1

Synonyms: hydroxy-delta-5-steroid dehydrogenase; 3 beta- and steroid delta-isomerase 1; 3BETAHSD; HSD3B; HSDB3; HSDB3A; SDR11E1; HSD3B1