Anti-hIST1/IST1 Antibody Picoband®

SKU: A05507-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: IST1

Specificity:

Background: IST1 homolog is a protein that in humans is encoded by the KIAA0174 gene. This gene encodes a protein with MIT-interacting motifs that interacts with components of endosomal sorting complexes required for transport (ESCRT). ESCRT functions in vesicle budding, such as that which occurs during membrane abscission in cytokinesis. There is a pseudogene for this gene on chromosome 19. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.

Immunogen: E.coli-derived human hIST1/IST1 recombinant protein (Position: E31-D331). Human IST1 shares 94% and 93.1% amino acid (aa) sequence identity with mouse and rat IST1, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Agromayor, M., Carlton, J. G., Phelan, J. P., Matthews, D. R., Carlin, L. M., Ameer-Beg, S., Bowers, K., Martin-Serrano, J. Essential role of hIST1 in cytokinesis. Molec. Biol. Cell 20: 1374-1387, 2009. 2. Bajorek, M., Morita, E., Skalicky, J. J., Morham, S. G., Babst, M., Sundquist, W. I. Biochemical analyses of human IST1 and its function in cytokinesis. Molec. Biol. Cell 20: 1360-1373, 2009. 3. Hartz, P. A. Personal Communication. Baltimore, Md. 6/23/2015.

Uniprot ID: P53990

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 40 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 9798

Protein Name: IST1 homolog

Gene Full Name: IST1 factor associated with ESCRT-III

Synonyms: hIST1; IST1; IST1 homolog; KIAA0174; OLC1