Anti-HAL Antibody Picoband®

SKU: A02612-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human
Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: HAL

Specificity:

Background: Histidine ammonia-lyase is a cytosolic enzyme catalyzing the first reaction in histidine catabolism, the nonoxidative deamination of L-histidine to trans-urocanic acid. Histidine ammonia-lyase defects cause histidinemia which is characterized by increased histidine and histamine and decreased urocanic acid in body fluids. Several transcript variants encoding different isoforms have been found for this gene.

Immunogen: E.coli-derived human HAL recombinant protein (Position: D65-R632). Human HALshares 95.2% and 95.4% amino acid (aa) sequence identity with mouse and rat HAL, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Kacser, H. K., Bulfield, G., Wallace, M. E. Histidinaemia mutant in the mouse. Nature 244: 77-79, 1973. 2. Kanarek, N., Keys, H. R., Cantor, J. R., Lewis, C. A., Chan, S. H., Kunchok, T., Abu-Remaileh, M., Freinkman, E., Schweitzer, L. D., Sabatini, D. M. Histidine catabolism is a major determinant of methotrexate sensitivity. Nature 559: 632-636, 2018. Note: Erratum: Nature 602: E17-E18, 2022. 3. Kawai, Y., Moriyama, A., Asai, K., Coleman-Campbell, C. M., Sumi, S., Morishita, H., Suchi, M. Molecular characterization of histidinemia: identification of four missense mutations in the histidase gene. Hum. Genet. 116: 340-346, 2005. Note: Erratum: Hum. Genet. 118: 531-532, 2005.

Uniprot ID: P42357

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 75 kDa

Calculated Molecular Weight: 73 kDa

Gene ID: 3034

Protein Name: Histidine ammonia-lyase

Gene Full Name: histidine ammonia-lyase

Synonyms: HAL; HIS; Histidase; histidine ammonia lyase; HSTD