Anti-HADH Antibody Picoband®

SKU: A03650-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.1-0.25 μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: HADH

Specificity:

Background: Hydroxyacyl-Coenzyme A dehydrogenase (HADH) is an enzyme which in humans is encoded by the HADH gene. This gene is a member of the 3-hydroxyacyl-CoA dehydrogenase gene family. The encoded protein functions in the mitochondrial matrix to catalyze the oxidation of straight-chain 3-hydroxyacyl-CoAs as part of the beta-oxidation pathway. Its enzymatic activity is highest with medium-chain-length fatty acids. Mutations in this gene cause one form of familial hyperinsulinemic hypoglycemia. The human genome contains a related pseudogene of this gene on chromosome 15.

Immunogen: E.coli-derived human HADH recombinant protein (Position: A43-K301). Human HADH shares 90.3% and 90.7% amino acid (aa) sequence identity with mouse and rat HADH, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Bennett, M. J., Weinberger, M. J., Kobori, J. A., Rinaldo, P., Burlina, A. B. Mitochondrial short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase deficiency: a new defect of fatty acid oxidation. Pediat. Res. 39: 185-188, 1996. 2. Bitar, K. G., Perez-Aranda, A., Bradshaw, R. A. Amino acid sequence of L-3-hydroxyacyl CoA dehydrogenase from pig heart muscle. FEBS Lett. 116: 196-198, 1980. 3. Clayton, P. T., Eaton, S., Aynsley-Green, A., Edginton, M., Hussain, K., Krywawych, S., Datta, V., Malingre, H. E. M., Berger, R., van den Berg, I. E. T. Hyperinsulinism in short-chain L-3-hydroxyacyl-CoA dehydrogenase deficiency reveals the importance of beta-oxidation in insulin secretion. J. Clin. Invest. 108: 457-465, 2001.

Uniprot ID: Q16836

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross reactivity with other proteins.

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 34 kDa

Calculated Molecular Weight: 52588 MW

Gene ID: 3033

Protein Name: Hydroxyacyl-coenzyme A dehydrogenase, mitochondrial

Gene Full Name: hydroxyacyl-CoA dehydrogenase

Synonyms: HAD; HADH; HADH1; HADHSC; HCDH; HHF4; M/SCHAD; SCHAD