Anti-GPNMB/Gpnmb Picoband® Antibody

SKU: A02439-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Mouse
Immunocytochemistry/Immunofluorescence, 2μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human, Mouse
ELISA, 0.1-0.5μg/ml, -

Gene Name: Gpnmb

Specificity: No cross reactivity with other proteins.

Background: Transmembrane glycoprotein NMB is a protein that in humans is encoded by the GPNMB gene. It is mapped to 6 B2.3; 6 23.82 cM. The protein encoded by this gene is a type I transmembrane glycoprotein which shows homology to the pMEL17 precursor, a melanocyte-specific protein. GPNMB shows expression in the lowly metastatic human melanoma cell lines and xenografts but does not show expression in the highly metastatic cell lines. GPNMB may be involved in growth delay and reduction of metastatic potential. Two transcript variants encoding different isoforms have been found for this gene.

Immunogen: E.coli-derived mouse GPNMB/Gpnmb recombinant protein (Position: R164-D564).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Lu, H., Wang, X., Pullen, M., Guan, H., Chen, H., Sahu, S., Zhang, B., Chen, H., Williams, R. W., Geisert, E. E., Lu, L., Jablonski, M. M. Genetic dissection of the Gpnmb network in the eye. Invest. Ophthal. Vis. Sci. 52: 4132-4142, 2011. 2. Weterman, M. A. J., Ajubi, N., van Dinter, I. M. R., Degen, W. G. J., van Muijen, G. N. P., Ruiter, D. J., Bloemers, H. P. J. Nmb, a novel gene, is expressed in low-metastatic human melanoma cell lines and xenografts. Int. J. Cancer 60: 73-81, 1995. 3. Yang, C.-F., Lin, S.-P., Chiang, C.-P., Wu, Y.-H., H'ng, W. S., Chang, C.-P., Chen, Y.-T., Wu, J.-Y. Loss of GPNMB causes autosomal-recessive amyloidosis cutis dyschromica in humans. Am. J. Hum. Genet. 102: 219-232, 2018.

Uniprot ID: Q99P91

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 64 kDa

Calculated Molecular Weight: 38779 MW

Gene ID: 93695

Protein Name: Transmembrane glycoprotein NMB

Gene Full Name: glycoprotein (transmembrane) nmb

Synonyms: glycoprotein nmb; HGFIN; NMB; PLCA3; Gpnmb