Anti-GPCR RDC1/CXCR-7/ACKR3 Antibody Picoband®

SKU: A02656-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: Flow Cytometry,IHC,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: ACKR3

Specificity:

Background: Atypical chemokine receptor 3 also known as C-X-C chemokine receptor type 7 (CXCR-7) and G-protein coupled receptor 159 (GPR159) is a protein that in humans is encoded by the ACKR3 gene. This gene encodes a member of the G-protein coupled receptor family. Although this protein was earlier thought to be a receptor for vasoactive intestinal peptide (VIP), it is now considered to be an orphan receptor, in that its endogenous ligand has not been identified. The protein is also a coreceptor for human immunodeficiency viruses (HIV). Translocations involving this gene and HMGA2 on chromosome 12 have been observed in lipomas.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human GPCR RDC1/CXCR-7/ACKR3, identical to the related mouse and rat sequences.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Berahovich, R. D., Zabel, B. A., Penfold, M. E. T., Lewen, S., Wang, Y., Miao, Z., Gan, L., Pereda, J., Dias, J., Slukvin, I. I., McGrath, K. E., Jaen, J. C., Schall, T. J. CXCR7 protein is not expressed on human or mouse leukocytes. J. Immun. 185: 5130-5139, 2010. 2. Boldajipour, B., Mahabaleshwar, H., Kardash, E., Reichman-Fried, M., Blaser, H., Minina, S., Wilson, D., Xu, Q., Raz, E. Control of chemokine-guided cell migration by ligand sequestration. Cell 132: 463-473, 2008. 3. Burns, J. M., Summers, B. C., Wang, Y., Melikian, A., Berahovich, R., Miao, Z., Penfold, M. E. T., Sunshine, M. J., Littman, D. R., Kuo, C. J., Wei, K., McMaster, B. E., Wright, K., Howard, M. C., Schall, T. J. A novel chemokine receptor for SDF-1 and I-TAC involved in cell survival, cell adhesion, and tumor development. J. Exp. Med. 203: 2201-2213, 2006.

Uniprot ID: P25106

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 41 kDa

Calculated Molecular Weight: 33310 MW

Gene ID: 57007

Protein Name: Atypical chemokine receptor 3

Gene Full Name: atypical chemokine receptor 3

Synonyms: Chemokine orphan receptor 1; CMKOR1; CXC R7; CXCR 7; CXCR7; G protein coupled receptor 159; GPR159; RDC 1; RDC1