Anti-GM-CSF/CSF2 Antibody Picoband®

SKU: PB9003

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Mouse

Applications: IHC,WB

Application Details: Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse
Western blot, 0.1-0.5μg/ml, Mouse

Gene Name: CSF2

Specificity: No cross reactivity with other proteins.

Background: GM-CSF, Granulocyte-macrophage colony-stimulating factor, is a protein secreted by macrophages, T cells, mast cells, endothelial cells, and fibroblasts. By fluorescence in situ hybridization, the GM-CSF gene is mapped to 5q31.1. GM-CSF is a cytokine that functions as a white blood cell growth factor. GM-CSF stimulates stem cells to produce granulocytes (neutrophils, eosinophils, and basophils) and monocytes. GM-CSF is an essential regulator of neutrophil function.

Immunogen: E.coli-derived mouse GM-CSF recombinant protein (Position: A18-K141). Mouse GM-CSF shares 54% and 70% amino acid (aa) sequences identity with human and rat GM-CSF, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Le Beau, M. M., Espinosa, R., III, Neuman, W. L., Stock, W., Roulston, D., Larson, R. A., Keinanen, M., Westbrook, C. A.Cytogenetic and molecular delineation of the smallest commonly deleted region of chromosome 5 in malignant myeloid diseases.Proc. Nat. Acad. Sci. 90: 5484-5488, 1993. 2. Park, B. K., Zhang, H., Zeng, Q., Dai, J., Keller, E. T., Giordano, T., Gu, K., Shah, V., Pei, L., Zarbo, R. J., McCauley, L., Shi, S., Chen, S., Wang, C.-Y.NF-kappa-B in breast cancer cells promotes osteolytic bone metastasis by inducing osteoclastogenesis via GM-CSF.Nature Med. 13: 62-69, 2007. 3. Uchida, K., Beck, D. C., Yamamoto, T., Berclaz, P.-Y., Abe, S., Staudt, M. K., Carey, B. C., Filippi, M.-D., Wert, S. E., Denson, L. A., Puchalski, J. T., Hauck, D. M., Trapnell, B. C.GM-CSF autoantibodies and neutrophil dysfunction in pulmonary alveolar proteinosis.New Eng. J. Med. 356: 567-579, 2007.

Uniprot ID: P01587

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 17 kDa

Calculated Molecular Weight: 16091 MW

Gene ID: 12981

Protein Name: Granulocyte-macrophage colony-stimulating factor

Gene Full Name: colony stimulating factor 2 (granulocyte-macrophage)

Synonyms: Colony stimulating factor; CSF; CSF2; GM CSF; GMCSF; GM-CSF; Molgramostin; Sargramostim