Anti-GLIS1 Antibody Picoband®

SKU: A12698-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: ELISA,Flow Cytometry,IF,IHC,ICC,WB

Application Details: Western blot, 0.25-0.5 μg/ml/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml/ml, Human

Gene Name: GLIS1

Specificity:

Background: Glis1 (Glis Family Zinc Finger 1) is gene encoding a Krüppel-like protein of the same name whose locus is found on Chromosome 1p32.3. GLIS1 is a GLI (MIM 165220)-related Kruppel-like zinc finger protein that functions as an activator and repressor of transcription.

Immunogen: E.coli-derived human GLIS1 recombinant protein (Position: M1-T620).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Kim, Y.-S., Lewandoski, M., Perantoni, A. O., Kurebayashi, S., Nakanishi, G., Jetten, A. M. Identification of Glis1, a novel Gli-related, Kruppel-like zinc finger protein containing transactivation and repressor functions. J. Biol. Chem. 277: 30901-30913, 2002. 2. Maekawa, M., Yamaguchi, K., Nakamura, T., Shibukawa, R., Kodanaka, I., Ichisaka, T., Kawamura, Y., Mochizuki, H., Goshima, N., Yamanaka, S. reprogramming of somatic cells is promoted by maternal transcription factor Glis1. Nature 474: 225-229, 2011. 3. Nakanishi, G., Kim, Y.-S., Nakajima, T., Jetten, A. M. Regulatory role for Kruppel-like zinc-finger protein Gli-similar 1 (Glis1) in PMA-treated and psoriatic epidermis J. Invest. Derm. 126: 49-60, 2006.

Uniprot ID: Q8NBF1

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 66 kDa

Calculated Molecular Weight:

Gene ID: 14897

Protein Name: Zinc finger protein GLIS1

Gene Full Name: GLIS family zinc finger 1

Synonyms: GLI similar 1; GLIS family zinc finger 1; GLIS1; Zinc finger protein GLIS1