Anti-GLI1 Antibody Picoband®

SKU: A00527-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -

Gene Name: GLI1

Specificity:

Background: GLI1, also known as GLI, is a protein originally isolated in human glioblastoma. The protein is encoded by GLI1 gene. It is mapped to 12q13.3. This gene is a member of a select group of cellular genes that are genetically altered in primary human tumors. GLI1 encodes a member of the GLI-Kruppel family of zinc finger proteins in the final steps of Hedgehog signaling in normal development and disease. The encoded transcription factor is activated by the sonic hedgehog signal transduction cascade and regulates stem cell proliferation. The activity and nuclear localization of this protein is negatively regulated by p53 in an inhibitory loop. Beside, various upstream patterning signals may be integrated by the Gli proteins to a coherent programme of neurogenesis.

Immunogen: E.coli-derived human GLI1 recombinant protein (Position: Y800-Q1057). Human GLI1 shares 79.8% amino acid (aa) sequence identity with mouse GLI1.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Kinzler KW,; Bigner SH; Bigner DD; Trent JM; Law ML; O'Brien SJ; Wong AJ; Vogelstein B. (April 1987). Identification of an amplified, highly expressed gene in a human glioma. Science 236 (4797): 70–3. 2. Ruiz i Altaba A. (June 1999). Gli proteins encode context-dependent positive and negative functions: implications for development and disease.. Development 126 (14): 3205–16. 3. Sasaki H,; Hui C; Nakafuku M; Kondoh H. (April 1997). A binding site for Gli proteins is essential for HNF-3beta floor plate enhancer activity in transgenics and can respond to Shh in vitro. Development 124 (7): 1313–22.

Uniprot ID: P08151

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 150 kDa

Calculated Molecular Weight:

Gene ID: 2735

Protein Name: Zinc finger protein GLI1

Gene Full Name: GLI family zinc finger 1

Synonyms: GLI family zinc finger 1; GLI; PAPA8; PPD1; GLI1