Anti-GARNL1/RALGAPA1 Antibody Picoband®

SKU: A08369-1

Size: 100 μg

Storage: At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml

Gene Name: RALGAPA1

Specificity:

Background: This gene encodes a major subunit of the RAL-GTPase activating protein. A similar protein in mouse binds E12, a transcriptional regulator of immunoglobulin genes. The mouse protein also functions in skeletal muscle by binding to the regulatory 14-3-3 proteins upon stimulation with insulin or muscle contraction. A pseudogene of this gene has been identified on chromosome 9.

Immunogen: E.coli-derived human GARNL1/RALGAPA1 recombinant protein (Position: S503-H1729). Human GARNL1/RALGAPA1 shares 95% amino acid (aa) sequence identity with both mouse and rat GARNL1/RALGAPA1.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Heng, J. I. T., Tan, S.-S. Cloning and characterization of GRIPE, a novel interacting partner of the transcription factor E12 in developing mouse forebrain. J. Biol. Chem. 277: 43152-43159, 2002. 2. Nagase, T., Ishikawa, K., Suyama, M., Kikuno, R., Miyajima, N., Tanaka, A., Kotani, H., Nomura, N., Ohara, O. Prediction of the coding sequences of unidentified human genes. XI. The complete sequences of 100 new cDNA clones from brain which code for large proteins in vitro. DNA Res. 5: 277-286, 1998. 3. Schwarzbraun, T., Vincent, J. B., Schumacher, A., Geschwind, D. H., Oliveira, J., Windpassinger, C., Ofner, L., Ledinegg, M. K., Kroisel, P. M., Wagner, K., Petek, E. Cloning, genomic structure, and expression profiles of TULIP1 (GARNL1), a brain-expressed candidate gene for 14q13-linked neurological phenotypes, and its murine homologue. Genomics 84: 577-586, 2004.

Uniprot ID: Q6GYQ0

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 250 kDa

Calculated Molecular Weight: 230 kDa

Gene ID: 253959

Protein Name: Ral GTPase-activating protein subunit alpha-1

Gene Full Name: Ral GTPase activating protein catalytic subunit alpha 1

Synonyms: DKFZp566D133; DKFZp667F074; GARNL1; GRIPE; KIAA0884; p240; RALGAPA1; Tuberin like protein 1; TULIP1