Anti-G3BP/G3BP1 Antibody Picoband®

SKU: A02199-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,ICC,IF,Flow Cytometry

Application Details: Western blot, 0.1-0.25 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: G3BP1

Specificity:

Background: Ras GTPase-activating protein-binding protein 1 is an enzyme that in humans is encoded by the G3BP1 gene. This gene encodes one of the DNA-unwinding enzymes which prefers partially unwound 3'-tailed substrates and can also unwind partial RNA/DNA and RNA/RNA duplexes in an ATP-dependent fashion. This enzyme is a member of the heterogeneous nuclear RNA-binding proteins and is also an element of the Ras signal transduction pathway. It binds specifically to the Ras-GTPase-activating protein by associating with its SH3 domain. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been determined.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human G3BP/G3BP1, which shares 64.7% amino acid (aa) sequence identity with mouse G3BP1.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Costa, M., Ochem, A., Staub, A., Falaschi, A. Human DNA helicase VIII: a DNA and RNA helicase corresponding to the G3BP protein, an element of the Ras transduction pathway. Nucleic Acids Res. 27: 817-821, 1999. 2. Parker, F., Maurier, F., Delumeau, I., Duchesne, M., Faucher, D., Debussche, L., Dugue, A., Schweighoffer, F., Tocque, B. A Ras-GTPase-activating protein SH3-domain-binding protein. Molec. Cell. Biol. 16: 2561-2569, 1996. 3. Tourriere, H., Chebli, K., Zekri, L., Courselaud, B., Blanchard, J. M., Bertrand, E., Tazi, J. The RasGAP-associated endoribonuclease G3BP assembles stress granules. J. Cell Biol. 160: 823-831, 2003.

Uniprot ID: Q13283

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 68 kDa

Calculated Molecular Weight: 31568 MW

Gene ID: 10146

Protein Name: Ras GTPase-activating protein-binding protein 1

Gene Full Name: G3BP stress granule assembly factor 1

Synonyms: G3BP; G3BP 1; G3BP1; HDH VIII