Anti-ErbB 3/ERBB3 Antibody Picoband®

SKU: PA1880

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: WB

Application Details: Western blot, 0.1-0.5μg/ml, Human

Gene Name: ERBB3

Specificity: No cross reactivity with other proteins.

Background: ERBB3 (V-erb-b2 avian erythroblastic leukemia viral oncogene homolog 3) also known as ONCOGENE ERBB3 or HER3, is an enzyme that in humans is encoded by the ERBB3 gene. This gene encodes a member of the epidermal growth factor receptor (EGFR) family of receptor tyrosine kinases. This membrane-bound protein has a neuregulin binding domain but not an active kinase domain. By in situ hybridization, Kraus et al. (1989) mapped the ERBB3 gene to chromosome 12q13. Carraway et al. (1994) demonstrated that ERBB3 is a receptor for heregulin and is capable of mediating HGL-stimulated tyrosine phosphorylation of itself. Most notably, EGFR and ErbB2 became markedly more promiscuous as the threshold was lowered, whereas ErbB3did not. Engelman et al. (2007) found that amplification of MET caused gefitinib resistance by driving ERBB3-dependent activation of phosphoinositide 3-kinase, a pathway though to be specific to EGFR/ERBB family receptors.

Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human ErbB 3.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Carraway, K. L., III, Sliwkowski, M. X., Akita, R., Platko, J. V., Guy, P. M., Nuijens, A., Diamonti, A. J., Vandlen, R. L., Cantley, L. C., Cerione, R. A. The erbB3gene product is a receptor for heregulin. J. Biol. Chem. 269: 14303-14306, 1994. 2. Engelman, J. A., Zejnullahu, K., Mitsudomi, T., Song, Y., Hyland, C., Park, J. O., Lindeman, N., Gale, C.-M., Zhao, X., Christensen, J., Kosaka, T., Holmes, A. J., Rogers, A. M., Cappuzzo, F., Mok, T., Lee, C., Johnson, B. E., Cantley, L. C, Janne, P. A. MET amplification leads to gefitinib resistance in lung cancer by activating ERBB3 signaling. Science 316: 1039-1043, 2007. 3. Kraus, M. H., Issing, W., Miki, T., Popescu, N. C., Aaronson, S. A. Isolation and characterization ofERBB3, a third member of the ERBB/epidermal growth factor receptor family: evidence for overexpression in a subset of human mammary tumors. Proc. Nat. Acad. Sci. 86: 9193-9197, 1989.

Uniprot ID: P21860

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 180 kDa

Calculated Molecular Weight: 148 kDa

Gene ID: 2065

Protein Name: Receptor tyrosine-protein kinase erbB-3

Gene Full Name: erb-b2 receptor tyrosine kinase 3

Synonyms: c erbB 3; c erbB3; ErbB 3; ERBB3; erbB3 S; HER3; LCCS2; MDA BF 1; p180 ErbB3; p45 sErbB3; p85 sErbB3