Anti-Eph receptor A3/EPHA3 Antibody Picoband®

SKU: A02872-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: EPHA3

Specificity:

Background: Ephrin Receptor EphA3, is also known as human embryo kinase(HEK) or Eph-like tyrosine kinase1(ETK1). Kinases that phosphorylate proteins on tyrosine residues(protein tyrosine kinases; PTKs), such as EPHA3, form a structurally related group of molecules that exhibit functional diversity. Genetic alterations that lead to the inappropriate activation or expression of PTKs may be oncogenic. Many growth factor receptors are PTKs, e.g., the receptors for epidermal growth factor(EGFR), platelet-derived growth factor(PDGFR1;PDGFR2), colony-stimulating factor-1(CSF1R), and stem cell growth factor. The EPHA3 gene is mapped to 3p11.2. Sequence comparison with other PTKs showed a high degree of homology with members of the EPH and ELK(EPHB1) families of receptor tyrosine kinases. Within axial nerves, establishment of discrete afferent and efferent pathways depends on coordinate signaling between coextending sensory and motor projections.

Immunogen: E.coli-derived human Eph receptor A3/EPHA3 recombinant protein (Position: D59-A514).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Boyd, A. W., Ward, L. D., Wicks, I. P., Simpson, R. J., Salvaris, E., Wilks, A., Welch, K., Loudovaris, M., Rockman, S., Busmanis, I. Isolation and characterization of a novel receptor-type protein tyrosine kinase (hek) from a human pre-B cell line. J. Biol. Chem. 267: 3262-3267, 1992. 2. Fox, G. M., Holst, P. L., Chute, H. T., Lindberg, R. A., Janssen, A. M., Basu, R., Welcher, A. A. cDNA cloning and tissue distribution of five human EPH-like receptor protein-tyrosine kinases. Oncogene 10: 897-905, 1995. 3. Gallarda, B. W., Bonanomi, D., Mueller, D., Brown, A., Alaynick, W. A., Andrews S. E., Lemke, G., Pfaff, S. L., Marquardt, T. Segregation of axial motor and sensory pathways via heterotypic trans-axonal signaling. Science 320: 233-236, 2008.

Uniprot ID: P29320

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 135 kDa

Calculated Molecular Weight:

Gene ID: 2042

Protein Name: Ephrin type-A receptor 3

Gene Full Name: EPH receptor A3

Synonyms: EPHA3; ETK; ETK1; HEK; TYRO4; EK4; hEK4; HEK; Human embryo kinase; Eph-like tyrosine kinase 1