Anti-EpCAM Antibody Picoband®

SKU: PB10059

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: ELISA,Flow Cytometry,IF,IHC,WB

Application Details: Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml
Immunofluorescence, 5μg/ml
Flow Cytometry (Fixed), 1-3μg/1x10⁶cells
ELISA, 0.1-0.5μg/ml

Gene Name: EPCAM

Specificity: No cross reactivity with other proteins.

Background: Epithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell–cell adhesion in epithelia. This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human EPCAM, different from the related mouse sequence by fifteen amino acids, and from the related rat sequence by sixteen amino acids.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Litvinov, Sergey; et al. (1994). Ep-CAM: a human epithelial antigen is a homophilic cell–cell adhesion molecule. The Journal of Cell Biology 125 (2): 437–46. 2. Maetzel, Dorothea; et al. (2009). Nuclear signalling by tumour-associated antigen EpCAM. Nature Cell Biology 11 (2): 162–71.

Uniprot ID: P16422

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 35-40 kDa

Calculated Molecular Weight: 34932 MW

Gene ID: 4072

Protein Name: Epithelial cell adhesion molecule

Gene Full Name: epithelial cell adhesion molecule

Synonyms: CD326; CO 17A; CO17 1A; EGP; EGP 2; EGP314; EGP34; EGP40; Ep CAM; EPCAM; Epithelial glycoprotein; Epithelial glycoprotein 314; ESA; GA733 2; hEGP 2; hEGP314; KS 1/4 antigen; KS1/4; KSA; M1S2; M4S1; MIC18; MK 1; TACST 1; TACSTD1; TROP1