Anti-EEA1 Antibody Picoband®

SKU: A02296-3

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: IF,IHC,ICC,WB

Application Details: Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml
Immunocytochemistry/Immunofluorescence, 5μg/ml

Gene Name: EEA1

Specificity: No cross reactivity with other proteins.

Background: The gene EEA1 encodes for the 1400 amino acid protein, Early Endosome Antigen 1. It localizes exclusively to early endosomes and has an important role in endosomal trafficking. EEA1 binds ly to the phospholipid phosphatidylinositol 3-phosphate through its C-terminal FYVE domain and forms ahomodimer through a coiled coil. Furthermore, EEA1 acts as a tethering molecule that couples vesicle docking with SNAREs such as N-ethylmaleimide sensitive fusion protein, bringing the endosomes physically closer and ultimately resulting in the fusion and delivery of endosomal cargo.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human EEA1, identical to the related mouse and rat sequences.

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Barysch SV, Aggarwal S, Jahn R, Rizzoli SO (Jun 2009). Sorting in early endosomes reveals connections to docking- and fusion-associated factors. Proceedings of the National Academy of Sciences of the United States of America. 106 (24): 9697–702. 2. Mishra A, Eathiraj S, Corvera S, Lambright DG (Jun 2010). Structural basis for Rab GTPase recognition and endosome tethering by the C2H2 zinc finger of Early Endosomal Autoantigen 1 (EEA1). Proceedings of the National Academy of Sciences of the United States of America. 107 (24): 10866–71.

Uniprot ID: Q15075

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 170 kDa

Calculated Molecular Weight: 162466 MW

Gene ID: 8411

Protein Name: Early endosome antigen 1

Gene Full Name: early endosome antigen 1

Synonyms: Cell and organelle markers; early endosome antigen 1; EEA1; Endosome Marker; MST105; MSTP105; ZFYVE2