Anti-CYP11B1/C11B2/CYP11B2 Antibody Picoband®

SKU: A03766-3

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: CYP11B1/2

Specificity:

Background: CYP11B1 shows close homology to the CYP11B2 gene, which encodes aldosterone synthase and is normally expressed only in the zona glomerulosa. Both genes map to chromosome 8q21. This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the mitochondrial inner membrane.

Immunogen: E.coli-derived human CYP11B1/C11B2/CYP11B2 recombinant protein (Position: M1-P491).

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Curnow, K. M., Slutsker, L., Vitek, J., Cole, T., Speiser, P. W., New, M. I., White, P. C., Pascoe, L. Mutations in the CYP11B1 gene causing congenital adrenal hyperplasia and hypertension cluster in exons 6, 7, and 8. Proc. Nat. Acad. Sci. 90: 4552-4556, 1993. 2. Clyne, C. D., Zhang, Y., Slutsker, L., Mathis, J. M., White, P. C., Rainey, W. E. Angiotensin II and potassium regulate human CYP11B2 transcription through common cis-elements. Molec. Endocr. 11: 638-649, 1997. 3. Ganapathipillai, S., Laval, G., Hoffman, I. S., Castejon, A. M., Nicod, J., Dick, B., Frey, F. J., Frey, B. M., Cubeddu, L. X., Ferrari, P. CYP11B2-CYP11B1 haplotypes associated with decreased 11-beta-hydroxylase activity. J. Clin. Endocr. Metab. 90: 1220-1225, 2005.

Uniprot ID: P15538/P19099

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 55 kDa

Calculated Molecular Weight:

Gene ID: 1584/1585

Protein Name: Ras-related protein Rab-39A/B

Gene Full Name: cytochrome P450 family 11 subfamily B member 1/2

Synonyms: Cytochrome P450 11B1; mitochondrial; CYPXIB1; Cytochrome P-450c11; Cytochrome P450C11; Steroid 11-beta-hydroxylase; Cytochrome P450 11B2; mitochondrial; Aldosterone synthase; ALDOS; Aldosterone-synthesizing enzyme; CYPXIB2; Cytochrome P-450Aldo; Cytochrome P-450C18; Steroid 18-hydroxylase