Anti-Cyclin-dependent-like kinase 5 Cdk5 Antibody Picoband®

SKU: PA1548

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,ICC/IF,Flow Cytometry

Application Details: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry(Fixed), 1-3 μg/1x10⁶ cells, Human

Gene Name: CDK5

Specificity: No cross reactivity with other proteins.

Background: CDK5, Cyclin-Dependent Kinase5, is also know as PSSALRE. The p34 (CDC2) protein kinase regulates important transitions in the eukaryotic cell cycle. The PSSALRE expression is in all human tissues and cell lines tested. The the CDK5 gene is mapped to chromosome 7q36. Cyclin-dependent kinase-5 is predominantly expressed in neurons where it phosphorylates both high molecular weight neurofilaments and microtubule-associated protein tau. CDK5 is required for proper development of the mammalian central nervous system. To be activated, CDK5 must associate with its regulatory subunit, p35 (CDK5R1). CDK5 can phosphorylate DARPP32 at threonine-75, converting it into an inhibitor of PKA.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Cdk5, identical to the related rat and mouse sequence.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Demetrick, D. J., Zhang, H., Beach, D. H.Chromosomal mapping of human CDK2, CDK4, and CDK5 cell cycle kinase genes.Cytogenet. Cell Genet. 66: 72-74, 1994. 2. Ohshima, T., Nagle, J. W., Pant, H. C., Joshi, J. B., Kozak, C. A., Brady, R. O., Kulkarni, A. B.Molecular cloning and chromosomal mapping of the mouse cyclin-dependent kinase 5 gene.Genomics 28: 585-588, 1995. 3. Bibb, J. A., Snyder, G. L., Nishi, A., Yan, Z., Meijer, L., Fienberg, A. A., Tsai, L.-H., Kwon, Y. T., Girault, J.-A., Czernik, A. J., Huganir, R. L., Hemmings, H. C., Jr., Nairn, A. C., Greengard, P.Phosphorylation of DARPP-32 by Cdk5 modulates dopamine signalling in neurons.Nature 402: 669-671, 1999.

Uniprot ID: Q00535

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 33 kDa

Calculated Molecular Weight: 33 kDa

Gene ID: 1020

Protein Name: Cyclin-dependent kinase 5

Gene Full Name: cyclin dependent kinase 5

Synonyms: CDK5; Cell division protein kinase 5; cyclin dependent kinase 5; PSSALRE; TPKII catalytic subunit