Anti-Cox6a2 Antibody Picoband®

SKU: A15662-1

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Mouse,Rat

Applications: WB

Application Details: Western blot, 0.25-0.5μg/ml, Mouse, Rat

Gene Name: Cox6a2

Specificity:

Background: Cytochrome c oxidase subunit VIa polypeptide 2 is a protein that in humans is encoded by the COX6A2 gene. Cytochrome c oxidase (COX), the terminal enzyme of the mitochondrial respiratory chain, catalyzes the electron transfer from reduced cytochrome c to oxygen. It is a heteromeric complex consisting of 3 catalytic subunits encoded by mitochondrial genes and multiple structural subunits encoded by nuclear genes. The mitochondrially-encoded subunits function in electron transfer, and the nuclear-encoded subunits may be involved in the regulation and assembly of the complex. This nuclear gene encodes polypeptide 2 (heart/muscle isoform) of subunit VIa, and polypeptide 2 is present only in striated muscles. Polypeptide 1 (liver isoform) of subunit VIa is encoded by a different gene, and is found in all non-muscle tissues. These two polypeptides share 66% amino acid sequence identity.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of mouse Cox6a2, which shares 66.7% and 100% amino acid (aa) sequence identity with human and rat Cox6a2, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Bachman, N. J., Riggs, P. K., Siddiqui, N., Makris, G. J., Womack, J. E., Lomax, M. I. Structure of the human gene (COX6A2) for the heart/muscle isoform of cytochrome c oxidase subunit VIa and its chromosomal location in humans, mice, and cattle. Genomics 42: 146-151, 1997. 2. Inoue, M., Uchino, S., Iida, A., Noguchi, S., Hayashi, S., Takahashi, T., Fujii, K., Komaki, H., Takeshita, E., Nonaka, I., Okada, Y., Yoshizawa, T., Van Lommel, L., Schuit, F., Goto, Y., Mimaki, M., Nishino, I. COX6A2 variants cause a muscle-specific cytochrome c oxidase deficiency. Ann. Neurol. 86: 193-202, 2019. 3. Quintens, R., Singh, S., Lemaire, K., De Bock, K., Granvik, M., Schraenen, A., Vroegrijk, I. O. C. M., Costa, V., Van Noten, P., Lambrechts, D., Lehnert, S., Van Lommel, L., and 10 others. Mice deficient in the respiratory chain gene Cox6a2 are protected against high-fat diet-induced obesity and insulin resistance. PLoS One 8: e56719, 2013. Note: Electronic Article.

Uniprot ID: P43023

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 11 kDa

Calculated Molecular Weight: 24145 MW

Gene ID: 12862

Protein Name: Cytochrome c oxidase subunit 6A2, mitochondrial

Gene Full Name: cytochrome c oxidase subunit 6A2

Synonyms: COX VIa M; COX6A; COX6A2; COX6AH; COXVIAH