Anti-Collagen Type II/COL2A1 Antibody Picoband®

SKU: A00517-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
ELISA, 0.1-0.5 μg/ml, -

Gene Name: COL2A1

Specificity:

Background: Collagen, type II, alpha 1 (primary osteoarthritis, spondyloepiphyseal dysplasia, congenital), also known as COL2A1, is a human gene that provides instructions for the production of the pro-alpha1(II) chain of type II collagen. This gene encodes the alpha-1 chain of type II collagen, a fibrillar collagen found in cartilage and the vitreous humor of the eye. Mutations in this gene are associated with achondrogenesis, chondrodysplasia, early onset familial osteoarthritis, SED congenita, Langer-Saldino achondrogenesis, Kniest dysplasia, Stickler syndrome type I, and spondyloepimetaphyseal dysplasia Strudwick type. In addition, defects in processing chondrocalcin, a calcium binding protein that is the C-propeptide of this collagen molecule, are also associated with chondrodysplasia. There are two transcripts identified for this gene.

Immunogen: E.coli-derived human Collagen Type II/COL2A1 recombinant protein (Position: G1217-A1241). Human COL2A1 shares 92% and 96% amino acid (aa) sequence identity with mouse and rat COL2A1, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Ahmad, N. N., Ala-Kokko, L., Knowlton, R. G., Jimenez, S. A., Weaver, E. J., Maguire, J. I., Tasman, W., Prockop, D. J. Stop codon in the procollagen II gene (COL2A1) in a family with the Stickler syndrome (arthro-ophthalmopathy). Proc. Nat. Acad. Sci. 88: 6624-6627, 1991. 2. Ahmad, N. N., Ala-Kokko, L., Knowlton, R. G., Weaver, E. J., Maguire, J. I., Tasman, W., Prockop, D. J. A stop codon in the gene for type II procollagen (COL2A1) causes one variant of arthro-ophthalmopathy (the Stickler syndrome). (Abstract) Am. J. Hum. Genet. 47 (suppl.): A206 only, 1990. 3. Ahmad, N. N., Dimascio, J., Knowlton, R. G., Tasman, W. S. Stickler syndrome: a mutation in the nonhelical 3-prime end of type II procollagen gene. Arch. Ophthal. 113: 1454-1457, 1995.

Uniprot ID: P02458

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity:

Isotype: IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 150-180 kDa

Calculated Molecular Weight: 142 kDa

Gene ID: 1280

Protein Name: Type II GnRH receptor

Gene Full Name: collagen type II alpha 1 chain

Synonyms: Alpha 1 type II collagen; ANFH; AOM; COL11A3; COL2A1; Col2α1; Collagen alpha 1(II) chain; Collagen Type II; SEDC