Anti-Collagen IV COL4A1 Antibody Picoband® (monoclonal, 3G3)

SKU: M01411

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: IF,IHC,WB

Application Details: Western blot, 0.1-0.5μg/ml
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml
Immunofluorescence, 2μg/ml

Gene Name: COL4A1

Specificity: No cross reactivity with other proteins.

Background: COL4A1, also known as ICH or Collagen alpha-1 (IV), is a protein that in humans is encoded by the COL4A1 gene. It is mapped to 13q34. This gene encodes the major type IV alpha collagen chain of basement membranes. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. COL4A1 binds to alpha-1/beta-1 integrin and inhibits migration, proliferation, and tube formation by endothelial cells. It is also a potential therapeutic candidate for targeting tumor angiogenesis.

Immunogen: E.coli-derived human Collagen IV recombinant protein (Position: G1445-T1669). Human Collagen IV shares 97% amino acid (aa) sequence identity with mouse Collagen IV.

Clonality: Monoclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Soininen R, Haka-Risku T, Prockop DJ, Tryggvason K (Jan 1988). Complete primary structure of the alpha 1-chain of human basement membrane (type IV) collagen. FEBS Lett 225 (1-2): 188–94. 2. Plaisier E, Gribouval O, Alamowitch S et al. (2007). COL4A1 mutations and hereditary angiopathy, nephropathy, aneurysms, and muscle cramps. N. Engl. J. Med. 357 (26): 2687–2695. 3. Wang, X., Harris, R. E., Bayston, L. J., Ashe, H. L. Type IV collagens regulate BMP signalling in Drosophila. Nature 455: 72-77, 2008.

Uniprot ID: P02462

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG1

Phospho_site:

Clone Number: Clone: 3G3

Observed Molecular Weight: 220 kDa

Calculated Molecular Weight:

Gene ID: 1282

Protein Name: Collagen alpha-1

Gene Full Name: collagen type IV alpha 1 chain

Synonyms: collagen type IV alpha 1 chain; BSVD; BSVD1s; PADMAL; RATOR; COL4A1