Anti-Claudin 1/CLDN1 Antibody

SKU: A01585-2

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: IHC,ICC,IF

Application Details: Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human

Gene Name: CLDN1

Specificity:

Background: Claudin-1 is a protein that in humans is encoded by the CLDN1 gene. Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. The protein encoded by this gene, a member of the claudin family, is an integral membrane protein and a component of tight junction strands. Loss of function mutations result in neonatal ichthyosis-sclerosing cholangitis syndrome.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human Claudin 1/CLDN1, identical to the related mouse and rat sequences.

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Baala, L., Hadj-Rabia, S., Hamel-Teillac, D., Hadchouel, M., Prost, C., Leal, S. M., Jacquemin, E., Sefiani, A., de Prost, Y., Courtois, G., Munnich, A., Lyonnet, S., Vabres, P. Homozygosity mapping of a locus for a novel syndromic ichthyosis to chromosome 3q27-q28. J. Invest. Derm. 119: 70-76, 2002. 2. Coyne, C. B., Gambling, T. M., Boucher, R. C., Carson, J. L., Johnson, L. G. Role of claudin interactions in airway tight junctional permeability. Am. J. Physiol. Lung Cell. Molec. Physiol. 285: L1166-L1178, 2003. 3. Dhawan, P., Singh, A. B., Deane, N. G., No, Y., Shiou, S.-R., Schmidt, C., Neff, J., Washington, M. K., Beauchamp, R. D. Claudin-1 regulates cellular transformation and metastatic behavior in colon cancer. J. Clin. Invest. 115: 1765-1776, 2005.

Uniprot ID: O95832

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 34 kDa

Calculated Molecular Weight: 34140 MW

Gene ID: 9076

Protein Name: Claudin-1

Gene Full Name: claudin 1

Synonyms: claudin 1; claudin1; CLD1; CLDN1; ILVASC; SEMP1