Anti-CD99 Antibody Picoband®

SKU: A01724-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human

Gene Name: CD99

Specificity:

Background: CD99 antigen (Cluster of differentiation 99), also known as MIC2 or single-chain type-1 glycoprotein, is a heavily O-glycosylated transmembrane protein that is encoded by the CD99 gene in humans. The protein encoded by this gene is a cell surface glycoprotein involved in leukocyte migration, T-cell adhesion, ganglioside GM1 and transmembrane protein transport, and T-cell death by a caspase-independent pathway. In addition, the encoded protein may have the ability to rearrange the actin cytoskeleton and may also act as an oncosuppressor in osteosarcoma. This gene is found in the pseudoautosomal region of chromosomes X and Y and escapes X-chromosome inactivation. There is a related pseudogene located immediately adjacent to this locus.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human CD99.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Banting, G. S., Pym, B., Goodfellow, P. N. Biochemical analysis of an antigen produced by both human sex chromosomes. EMBO J. 4: 1967-1972, 1985. 2. Bernard, A., Aubrit, A., Raynal, B., Phan, D., Boumsell, L. A T cell surface molecule different from CD2 is involved in spontaneous rosette formation with erythrocytes. J. Immun. 140: 1802-1807, 1988. 3. Bixel, M. G., Li, H., Petri, B., Khandoga, A. G., Khandoga, A., Zarbock, A., Wolburg-Buchholz, K., Wolburg, H., Sorokin, L., Zeuschner, D., Maerz, S., Butz, S., Krombach, F., Vestweber, D. CD99 and CD99L2 act at the same site as, but independently of, PECAM-1 during leukocyte diapedesis. Blood 116: 1172-1184, 2010.

Uniprot ID: P14209

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 28 kDa

Calculated Molecular Weight: 57954 MW

Gene ID: 4267

Protein Name: CD99 antigen

Gene Full Name: CD99 molecule (Xg blood group)

Synonyms: 12E7; CD99; CD99 antigen; CD99 molecule; E2 antigen; MIC2; MIC2X; MIC2Y; Protein MIC2; T cell surface glycoprotein E2