Anti-CD79a Antibody Picoband® (monoclonal, 4G4)

SKU: M01047-3

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: IHC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human

Gene Name: CD79A

Specificity: No cross reactivity with other proteins.

Background: Cluster of differentiation CD79A also known as B-cell antigen receptor complex-associated protein alpha chain and MB-1 membrane glycoprotein, is a protein that in humans is encoded by the CD79A gene. It is mapped to 19q13.2. CD79A is a membrane protein with an extracellular immunoglobulin domain, a single span transmembrane region and a short cytoplasmic domain. Genetic deletion of the transmembrane exon of CD79A results in loss of CD79A protein and a complete block of B cell development at the pro to pre B cell transition. Similarly, humans with homozygous splice variants in CD79A predicted to result in loss of the transmembrane region and a truncated or absent protein display agammaglobulinemia and no peripheral B cells.

Immunogen: E.coli-derived human CD79a recombinant protein (Position: T121-P226). Human CD79a shares 91% amino acid (aa) sequence identity with mouse CD79a.

Clonality: Monoclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500μg/ml.

Reference: 1. Pelanda R, Braun U, Hobeika E, Nussenzweig MC, Reth M (2002). B cell progenitors are arrested in maturation but have intact VDJ recombination in the absence of Ig-alpha and Ig-beta. J. Immunol. 169 (2): 865–72. 2. Minegishi Y, Coustan-Smith E, Rapalus L, Ersoy F, Campana D, Conley ME (1999). Mutations in Igalpha (CD79a) result in a complete block in B-cell development.. The Journal of Clinical Investigation 104 (8): 1115–21. 3. Wang Y, Kanegane H, Sanal O, Tezcan I, Ersoy F, Futatani T, Miyawaki T (2002). Novel Ig a (CD79a) gene mutation in a Turkish patient with B cell-deficient agammaglobulinemia. American Journal of Medical Genetics 336: 333–336.

Uniprot ID: P11912

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG1

Phospho_site:

Clone Number: Clone: 4G4

Observed Molecular Weight: 44 kDa

Calculated Molecular Weight:

Gene ID: 973

Protein Name: B-cell antigen receptor complex-associated protein alpha chain

Gene Full Name: CD79a molecule

Synonyms: CD79A; Ig alpha; IGA; MB 1; MB 1 membrane glycoprotein; MB1; Surface IgM associated protein