Anti-CD28 Antibody Picoband®

SKU: A00065-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: WB,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: CD28

Specificity:

Background: The protein encoded by this gene is essential for T-cell proliferation and survival, cytokine production, and T-helper type-2 development. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Immunogen: E.coli-derived human CD28 recombinant protein (Position: Q15-D214).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Akieda, Y., Wakamatsu, E., Nakamura, T., Ishida, Y., Ogawa, S., Abe, R. Defects in regulatory T cells due to CD28 deficiency induce a qualitative change of allogeneic immune response in chronic graft-versus-host disease. J. Immun. 194: 4162-4174, 2015. 2. Andres, P. G., Howland, K. C., Nirula, A., Kane, L. P., Barron, L., Dresnek, D., Sadra, A., Imboden, J., Weiss, A., Abbas, A. K. Distinct regions in the CD28 cytoplasmic domain are required for T helper type 2 differentiation. Nature Immun. 5: 435-442, 2004. 3. Aruffo, A., Seed, B. Molecular cloning of a CD28 cDNA by a high-efficiency COS cell expression system. Proc. Nat. Acad. Sci. 84: 8573-8577, 1987.

Uniprot ID: P10747

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 60 kDa

Calculated Molecular Weight: 25283 MW

Gene ID: 940

Protein Name: T-cell-specific surface glycoprotein CD28

Gene Full Name: CD28 molecule

Synonyms: CD28; CD28 molecule; Tp44