Anti-CBLIF Antibody Picoband®

SKU: A00038-1

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: CBLIF

Specificity:

Background: This gene is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12. Vitamin B12 is necessary for erythrocyte maturation and mutations in this gene may lead to congenital pernicious anemia.

Immunogen: E.coli-derived human CBLIF recombinant protein (Position: E128-Y417).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Andersen, C. B. F., Madsen, M., Storm, T., Moestrup, S. K., Andersen, G. R. Structural basis for receptor recognition of vitamin-B12-intrinsic factor complexes. Nature 464: 445-448, 2010. 2. Fernandes, M., Poirier, C., Lespinasse, F., Carle, G. F. The mouse homologs of human GIF, DDB1, and CFL1 genes are located on chromosome 19. Mammalian Genome 9: 339-342, 1998. 3. Gordon, M. M., Brada, N., Remacha, A., Badell, I., del Rio, E., Baiget, M., Santer, R., Quadros, E. V., Rothenberg, S. P., Alpers, D. H. A genetic polymorphism in the coding region of the gastric intrinsic factor gene (GIF) is associated with congenital intrinsic factor deficiency. Hum. Mutat. 23: 85-91, 2004.

Uniprot ID: P27352

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 52 kDa

Calculated Molecular Weight: 87335 MW

Gene ID: 2694

Protein Name: Cobalamin binding intrinsic factor

Gene Full Name: cobalamin binding intrinsic factor

Synonyms: CBLIF; GIF; IFMH; Intrinsic factor; IF; INF; TCN3