Anti-BMAL1/ARNTL Antibody Picoband®

SKU: A00260-3

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,ICC/IF,IHC,WB

Application Details: Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
ELISA, 0.1-0.5μg/ml, -

Gene Name: ARNTL

Specificity: No cross reactivity with other proteins.

Background: Aryl hydrocarbon receptor nuclear translocator-like protein 1 is protein that in humans is encoded by the ARNTL gene. The protein encoded by this gene is a basic helix-loop-helix protein that forms a heterodimer with CLOCK. This heterodimer binds E-box enhancer elements upstream of Period (PER1, PER2, PER3) and Cryptochrome (CRY1, CRY2) genes and activates transcription of these genes. PER and CRY proteins heterodimerize and repress their own transcription by interacting in a feedback loop with CLOCK/ARNTL complexes. Defects in this gene have been linked to infertility, problems with gluconeogenesis and lipogenesis, and altered sleep patterns. Several transcript variants encoding different isoforms have been found for this gene.

Immunogen: E.coli-derived human BMAL1/ARNTL recombinant protein (Position: R116-E447).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Hatanaka F, Matsubara C, Myung J, Yoritaka T, Kamimura N, Tsutsumi S, Kanai A, Suzuki Y, Sassone-Corsi P, Aburatani H, Sugano S,Takumi T (Dec 2010). Genome-wide profiling of the core clock protein BMAL1 targets reveals a strict relationship with metabolism. Molecular and Cellular Biology. 30 (24): 5636–5648. 2. Pappa KI, Gazouli M, Anastasiou E, Iliodromiti Z, Antsaklis A, Anagnou NP (Feb 2013). The major circadian pacemaker ARNT-like protein-1 (BMAL1) is associated with susceptibility to gestational diabetes mellitus. Diabetes Research and Clinical Practice. 99 (2): 151–7. 3. Richards J, Diaz AN, Gumz ML (Oct 2014). Clock genes in hypertension: novel insights from rodent models. Blood Pressure Monitoring. 19 (5): 249–54.

Uniprot ID: O00327

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 75 kDa

Calculated Molecular Weight: 69 kDa

Gene ID: 406

Protein Name: Basic helix-loop-helix ARNT-like protein 1

Gene Full Name: basic helix-loop-helix ARNT like 1

Synonyms: ARNT3; ARNTL; bHLH PAS protein JAP3; bHLHe5; BMAL1; BMAL1c; Brain and muscle ARNT like 1; JAP3; Member of PAS protein 3; MOP3; PASD3; TIC