Anti-ATP5F1B Antibody Picoband®

SKU: A32270-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: WB,IHC,ICC,IF,Flow Cytometry,ELISA

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10^6 cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: ATP5F1B

Specificity:

Background: This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the beta subunit of the catalytic core.

Immunogen: E.coli-derived human ATP5F1B recombinant protein (Position: Q123-S529).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Kim, K., Kim, S.-H., Kim, J., Kim, H., Yim, J. Glutathione S-transferase omega 1 activity is sufficient to suppress neurodegeneration in a Drosophila model of Parkinson disease. J. Biol. Chem. 287: 6628-6641, 2012. 2. Kudoh, J., Minoshima, S., Fukuyama, R., Maekawa, M., Neckelmann, N., Wallace, D. C., Shimizu, Y., Shimizu, N. Assignment of ATP synthase beta subunit (ATPMB) gene to the p13-qter region of human chromosome 12. (Abstract) Cytogenet. Cell Genet. 51: 1026 only, 1989. 3. Martinez, L. O., Jacquet, S., Esteve, J.-P., Rolland, C., Cabezon, E., Champagne, E., Pineau, T., Georgeaud, V., Walker, J. E., Terce, F., Collet, X., Perret, B., Barbaras, R. Ectopic beta-chain of ATP synthase is an apolipoprotein A-I receptor in hepatic HDL endocytosis. Nature 421: 75-79, 2003.

Uniprot ID: P06576

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 54 kDa

Calculated Molecular Weight: 14377 MW

Gene ID: 506

Protein Name: ATP synthase F

Gene Full Name: ATP synthase F1 subunit beta

Synonyms: ATP5B; ATPB; ATPMB; ATPSB; Cell and organelle markers; Mitochondria Marker; Mitochondrial Marker; Mitochondrion Marker