Anti-Asparagine synthetase/ASNS Antibody Picoband®

SKU: A03302-2

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: ELISA,Flow Cytometry,IF,IHC,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human, Mouse, Rat
Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human
Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: ASNS

Specificity:

Background: The protein encoded by this gene is involved in the synthesis of asparagine. This gene complements a mutation in the temperature-sensitive hamster mutant ts11, which blocks progression through the G1 phase of the cell cycle at nonpermissive temperature. Alternatively spliced transcript variants have been described for this gene.

Immunogen: E.coli-derived human Asparagine synthetase/ASNS recombinant protein (Position: M1-D531).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Abhyankar, A., Lamendola-Essel, M., Brennan, K., Giordano, J. L., Esteves, C., Felice, V., Wapner, R., Jobanputra, V. Clinical whole exome sequencing from dried blood spot identifies novel genetic defect underlying asparagine synthetase deficiency. Clin. Case Rep. 6: 200-205, 2018. 2. Arfin, S. M., Cirullo, R. E., Arredondo-Vega, F. X., Smith, M. Assignment of the structural gene for asparagine synthetase to human chromosome 7. Somat. Cell Genet. 9: 517-531, 1983. 3. Greco, A., Ittmann, M., Barletta, C., Basilico, C., Croce, C. M., Cannizzaro, L. A., Huebner, K. Chromosomal localization of human genes required for G(1) progression in mammalian cells. Genomics 4: 240-245, 1989.

Uniprot ID: P08243

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 64 kDa

Calculated Molecular Weight:

Gene ID: 440

Protein Name: Asparagine synthetase [glutamine-hydrolyzing]

Gene Full Name: asparagine synthetase (glutamine-hydrolyzing)

Synonyms: ASNS; asparagine synthetase; TS11