Anti-Aldolase/ALDOA Antibody Picoband® (monoclonal, 6H8)

SKU: M05022-2

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human

Applications: WB,IHC,ICC,IF,Flow Cytometry

Application Details: Western blot, 0.25-0.5μg/ml, Human
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-μ米g/1x10⁶ cells, Human

Gene Name: ALDOA

Specificity:

Background: Aldolase A (ALDOA, or ALDA), also known as fructose-bisphosphate aldolase, is an enzyme that in humans is encoded by the ALDOA gene on chromosome 16. This gene encodes a member of the class I fructose-bisphosphate aldolase protein family. The encoded protein is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde 3-phosphate and dihydroxyacetone phosphate. Three aldolase isozymes (A, B, and C), encoded by three different genes, are differentially expressed during development. Mutations in this gene have been associated with Glycogen Storage Disease XII, an autosomal recessive disorder associated with hemolytic anemia. Disruption of this gene also plays a role in the progression of multiple types of cancers. Related pseudogenes have been identified on chromosomes 3 and 10.

Immunogen: E.coli-derived human Aldolase/ALDOA recombinant protein (Position: E50-Y364).

Clonality: Monoclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl and 0.2mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Du, S; Guan, Z; Hao, L; Song, Y; Wang, L; Gong, L; Liu, L; Qi, X; Hou, Z; Shao, S (2014). Fructose-bisphosphate aldolase a is a potential metastasis-associated marker of lung squamous cell carcinoma and promotes lung cell tumorigenesis and migration. PLOS ONE. 9 (1): e85804. 2. Yao, DC; Tolan, DR; Murray, MF; Harris, DJ; Darras, BT; Geva, A; Neufeld, EJ (15 March 2004). Hemolytic anemia and severe rhabdomyolysis caused by compound heterozygous mutations of the gene for erythrocyte/muscle isozyme of aldolase, ALDOA(Arg303X/Cys338Tyr). Blood. 103 (6): 2401–3. 3. St-Jean, M; Izard, T; Sygusch, J (11 May 2007). A hydrophobic pocket in the active site of glycolytic aldolase mediates interactions with Wiskott-Aldrich syndrome protein. The Journal of Biological Chemistry. 282 (19): 14309–15.

Uniprot ID: P04075

Host: Mouse

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Mouse IgG2b

Phospho_site:

Clone Number: Clone: 6H8

Observed Molecular Weight: 39 kDa

Calculated Molecular Weight:

Gene ID: 226

Protein Name: Fructose-bisphosphate aldolase A

Gene Full Name: aldolase, fructose-bisphosphate A

Synonyms: ALDOA; Aldolase A