Anti-ALDH1A3 Antibody Picoband®

SKU: A03030

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: Flow Cytometry,IF,IHC,ICC,WB

Application Details: Western blot, 0.1-0.5μg/ml, Human, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Rat
Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human

Gene Name: ALDH1A3

Specificity: No cross reactivity with other proteins.

Background: Aldehyde dehydrogenase 1 family, member A3, also known as ALDH1A3 or retinaldehyde dehydrogenase 3 (RALDH3), is an enzyme that in humans is encoded by the ALDH1A3 gene. Aldehyde dehydrogenase isozymes are thought to play a major role in the detoxification of aldehydes generated by alcohol metabolism and lipid peroxidation. The enzyme encoded by this gene uses retinal as a substrate, either in a free or a cellular retinol-binding protein form. Mutations in this gene have been associated with microphthalmia, isolated 8, and expression changes have also been detected in tumor cells. Alternative splicing results in multiple transcript variants.

Immunogen: E.coli-derived human ALDH1A3 recombinant protein (Position: N37-K154). Human ALDH1A3 shares 89% and 87.3% amino acid (aa) sequence identity with mouse and rat ALDH1A3, respectively.

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Nishimura M, Yoshitsugu H, Naito S, Hiraoka I (2002). Evaluation of gene induction of drug-metabolizing enzymes and transporters in primary culture of human hepatocytes using high-sensitivity real-time reverse transcription PCR.. Yakugaku Zasshi. 122 (5): 339–61. 2. Rexer BN, Zheng WL, Ong DE (2001). Retinoic acid biosynthesis by normal human breast epithelium is via aldehyde dehydrogenase 6, absent in MCF-7 cells.. Cancer Res. 61 (19): 7065–70. 3. Yoshida A, Rzhetsky A, Hsu LC, Chang C (1998). Human aldehyde dehydrogenase gene family.. Eur. J. Biochem. 251 (3): 549–57.

Uniprot ID: P47895

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 50-56 kDa

Calculated Molecular Weight: 56 kDa

Gene ID: 220

Protein Name: Retinaldehyde dehydrogenase 3

Gene Full Name: aldehyde dehydrogenase 1 family member A3

Synonyms: Aldehyde dehydrogenase 6; ALDH1A3; ALDH1A6; ALDH6; RALDH 3; RALDH3; Retinaldehyde dehydrogenase 3