Anti-Acetyl Coenzyme A Carboxylase/ACACB Antibody Picoband®

SKU: A03668-2

Size: 100 μg

Storage: Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

Form: Lyophilized

Reactivity: Human,Mouse,Rat

Applications: Flow Cytometry,IHC,WB

Application Details: Western blot, 0.25-0.5μg/ml, Mouse, Rat
Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Mouse, Rat
Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human

Gene Name: ACACB

Specificity: No cross reactivity with other proteins.

Background: Acetyl-CoA carboxylase 2 also known as ACC-beta or ACC2 is an enzyme that in humans is encoded by the ACACB gene. It is mapped to 12q24.11. Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. ACC-beta is thought to control fatty acid oxidation by means of the ability of malonyl-CoA to inhibit carnitine-palmitoyl-CoA transferase I, the rate-limiting step in fatty acid uptake and oxidation by mitochondria. ACC-beta may be involved in the regulation of fatty acid oxidation, rather than fatty acid biosynthesis. There is evidence for the presence of two ACC-beta isoforms.

Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human Acetyl Coenzyme A Carboxylase/ACACB, which shares 64.7% amino acid (aa) sequence identity with mouse Acetyl Coenzyme A Carboxylase/ACACB.

Clonality: Polyclonal

Contents: Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.

Purification: Immunogen affinity purified.

Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

Reference: 1. Widmer J, Fassihi KS, Schlichter SC, Wheeler KS, Crute BE, King N, Nutile-McMenemy N, Noll WW, Daniel S, Ha J, Kim KH, Witters LA (June 1996). Identification of a second human acetyl-CoA carboxylase gene. The Biochemical Journal. 316 ( Pt 3) (3): 915–22. 2. Corbett JW, Harwood JH (November 2007). Inhibitors of mammalian acetyl-CoA carboxylase. Recent Patents on Cardiovascular Drug Discovery. 2 (3): 162–80. 3. Olson DP, Pulinilkunnil T, Cline GW, Shulman GI, Lowell BB (April 2010). Gene knockout of Acc2 has little effect on body weight, fat mass, or food intake. Proceedings of the National Academy of Sciences of the United States of America. 107 (16): 7598–603.

Uniprot ID: O00763

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate: This is the unconjugated antibody. It is also available with 10 conjugation options, including Biotin, Cy3, Fluoro488, Fluoro550, Fluoro594, FITC, HRP, APC, PE, and Fluoro647. A carrier-free (BSA-free) version is also available. Additional charges may apply for some options. Please contact us for a quote

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 277 kDa

Calculated Molecular Weight: 28461 MW

Gene ID: 32

Protein Name: Acetyl-CoA carboxylase 2

Gene Full Name: acetyl-CoA carboxylase beta

Synonyms: acetyl-CoA carboxylase beta; ACC2; ACCB; HACC275; ACACB; ACACbeta; ACC-beta; ACCbeta