Anti-ACACA Antibody Picoband®

SKU: A01802-3

Size: 100 μg

Storage: At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

Form: Lyophilized

Reactivity: Human

Applications: ELISA,Flow Cytometry,IF,ICC,WB

Application Details: Western blot, 0.25-0.5 μg/ml, Human
Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
ELISA, 0.1-0.5 μg/ml, -

Gene Name: ACACA

Specificity:

Background: Acetyl-CoA carboxylase 1 also known as ACC-alpha or ACCa is an enzyme that in humans is encoded by the ACACA gene. Acetyl-CoA carboxylase (ACC) is a complex multifunctional enzyme system. ACC is a biotin-containing enzyme which catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the rate-limiting step in fatty acid synthesis. There are two ACC forms, alpha and beta, encoded by two different genes. ACC-alpha is highly enriched in lipogenic tissues. The enzyme is under long term control at the transcriptional and translational levels and under short term regulation by the phosphorylation/dephosphorylation of targeted serine residues and by allosteric transformation by citrate or palmitoyl-CoA. Multiple alternatively spliced transcript variants divergent in the 5' sequence and encoding distinct isoforms have been found for this gene.

Immunogen: E.coli-derived human ACACA recombinant protein (Position: D31-R2336).

Clonality: Polyclonal

Contents: Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

Purification: Immunogen affinity purified.

Reconstitution: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Reference: 1. Abu-Elheiga, L., Brinkley, W. R., Zhong, L., Chirala, S. S., Woldegiorgis, G., Wakil, S. J. The subcellular localization of acetyl-CoA carboxylase 2. Proc. Nat. Acad. Sci. 97: 1444-1449, 2000. 2. Abu-Elheiga, L., Jayakumar, A., Baldini, A., Chirala, S. S., Wakil, S. J. Human acetyl-CoA carboxylase: characterization, molecular cloning, and evidence for two isoforms. Proc. Nat. Acad. Sci. 92: 4011-4015, 1995. 3. Abu-Elheiga, L., Matzuk, M. M., Kordari, P., Oh, W., Shaikenov, T., Gu, Z., Wakil, S. J. Mutant mice lacking acetyl-CoA carboxylase 1 are embryonically lethal. Proc. Nat. Acad. Sci. 102: 12011-12016, 2005.

Uniprot ID: Q13085

Host: Rabbit

Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

Conjugate:

Cross Reactivity: No cross-reactivity with other proteins.

Isotype: Rabbit IgG

Phospho_site:

Clone Number:

Observed Molecular Weight: 266 kDa

Calculated Molecular Weight:

Gene ID: 31

Protein Name: Acetyl-CoA carboxylase 1

Gene Full Name: acetyl-CoA carboxylase alpha

Synonyms: ACAC; ACACA; ACC; ACC1; ACCA; Acetyl CoA carboxylase 1